Evidence map›Paper›PMID 42493643›Full record

ArticleEMBO reports2026

cPRC1.2 and CTCF-mediated transition from poised to active chromatin loops at bivalent genes.

Aflah Hanafiah, Zhuangzhuang Geng, Tingting Liu, Yen Teng Tai, Wenjie Cai, Qiang Wang, Neil Christensen, Yan Liu, Feng Yue, Zhonghua Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Aflah Hanafiah *Department of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA.ORCID http://orcid.org/0000-0002-3221-3559
Zhuangzhuang Geng *Department of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA.
Tingting LiuDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
Yen Teng TaiDepartment of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA.ORCID http://orcid.org/0000-0002-0764-5034
Wenjie CaiDepartment of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.ORCID http://orcid.org/0000-0002-5352-8779
Qiang WangDepartment of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA.
Neil ChristensenDepartment of Pathology and Laboratory Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA.
Yan LiuCenter for Cancer Genomics, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
Feng YueDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA. Yue@Northwestern.edu.ORCID http://orcid.org/0000-0002-7954-5462
Zhonghua GaoDepartment of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA. gaozhonghua@suat-sz.edu.cn.ORCID http://orcid.org/0000-0001-9884-5818

Funding

Epigenetic regulation of cell identity by PRC1 complexesR35GM133496 · NIGMS · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI GAO, ZHONGHUA · 2019 to 2023
$2.1M
HHS | NIH | National Institute of General Medical Sciences (NIGMS) R35GM133496
6 · The paper itself

Abstract

Polycomb Repressive Complex 1 (PRC1) and CCCTC-binding factor (CTCF) are critical regulators of 3D chromatin architecture that influence cellular transcriptional programs. Although the role of CTCF in chromatin organization is well-known, the involvement of PRC1 is less understood. In this study, we identify an unexpected role for the canonical Pcgf2-containing PRC1 complex (cPRC1.2) in activating bivalent genes. Hi-C revealed that cPRC1.2 forms chromatin loops at bivalent promoters, rendering them poised for activation. Pcgf2 deletion disrupts cPRC1.2 loops and impairs the transcriptional induction of crucial target genes necessary for neuronal differentiation. Furthermore, we identify CTCF enrichment at cPRC1.2 loop anchors and at Polycomb group (PcG) bodies, suggesting that PRC1 and CTCF cooperatively regulate chromatin loops. Through virtual 4C and other genomic analyses, we discover that establishing neuronal progenitor cell (NPC) identity involves a switch from cPRC1.2-mediated chromatin loops to CTCF-mediated active loops. Our results suggest a novel mechanism by which pre-formed PRC1 loops at lineage-specific genes maintain a poised state for subsequent CTCF-mediated active loops and gene activation in cell fate transitions.

Indexed as

CCCTC-Binding FactorChromatinPolycomb Repressive Complex 1Repressor ProteinsAnimalsCell DifferentiationMiceNeural Stem CellsNeuronsPromoter Regions, GeneticProtein BindingCCCTC-Binding FactorChromatinCtcf protein, mousePolycomb Repressive Complex 1Repressor Proteins

Identifiers

PMID42493643
PMCPMC13554221

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.