ArticlePhotosynthesis research2026
Improved state‑transition analysis in tobacco lines differing in isoprene emission via spectral‑retrieval separation of PSI and PSII fluorescence at physiological temperature.
Article in Photosynthesis research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
State transition (ST) is a key regulatory mechanism that balances excitation energy between photosystem II (PSII) and photosystem I (PSI). Although its general principles have been known for decades, methodological constraints have limited precise quantification of ST kinetics and of the associated changes in photosystem absorption cross sections. We present a PCA‑assisted fluorescence spectral analysis that enables in vivo separation of PSI and PSII chlorophyll fluorescence at physiological temperatures. Applied to intact Nicotiana tabacum leaves undergoing ST, this method provides more accurate estimates of both the rates and the extent of the process than earlier approaches. The half‑time of the State 1 to State 2 (St1→St2) transition was approximately 1 min, nearly twice as fast as values inferred from traditional F
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