Evidence map›Paper›PMID 42489909›Full record

ArticleArchives of microbiology2026

Functional characterization of Hul6 reveals a role in iron-dependent metabolism in Schizosaccharomyces pombe.

Rongrong Li, Mengdan Zhu, Yusra Ashfaq, Yu Zhu, Chuanhai Fu, Yasir Nawaz, Ying Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rongrong LiJiangsu Key Laboratory for Microbes and Genomics, Department of Microbiology, School of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
Mengdan ZhuAnhui Key Laboratory of Chemical Biology and New Quality Medicine &, School of Life Sciences, Hefei National Research Center for Interdisciplinary Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Yusra AshfaqJiangsu Key Laboratory for Microbes and Genomics, Department of Microbiology, School of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
Yu ZhuJiangsu Key Laboratory for Microbes and Genomics, Department of Microbiology, School of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
Chuanhai FuAnhui Key Laboratory of Chemical Biology and New Quality Medicine &, School of Life Sciences, Hefei National Research Center for Interdisciplinary Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Yasir NawazJiangsu Key Laboratory for Microbes and Genomics, Department of Microbiology, School of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
Ying HuangJiangsu Key Laboratory for Microbes and Genomics, Department of Microbiology, School of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China. y_shang_hai@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Iron is essential for all organisms. In Schizosaccharomyces pombe, the GATA-binding repressor Fep1 and CCAAT-binding repressor Php4 control the expression of iron uptake and iron utilization genes, respectively, and mutually repress each other. We have shown that the E3 ligase Hul6 regulates Php4 target genes through Php4 degradation. Here, we further investigated Hul6 targets. RNA-seq analysis identified 327 differentially expressed genes (DEGs). GO analysis revealed upregulation of iron uptake genes and downregulation of genes involved in iron-dependent metabolism including mitochondrial oxidative phosphorylation (OXPHOS), suggesting an iron starvation-like response in Δhul6 cells. Promoter analysis identified GATA and CCAAT motifs in DEGs, suggesting potential direct targets for Fep1 and Php4, respectively. Furthermore, Δhul6 cells showed reduction in oxygen consumption and mitochondrial membrane potential, further supporting that Hul6 regulates OXPHOS. Our findings extend previous studies and further support a role for Hul6 in the control of iron-dependent metabolism in S. pombe.

Indexed as

IronSchizosaccharomycesSchizosaccharomyces pombe ProteinsUbiquitin-Protein LigasesCCAAT-Binding FactorGATA Transcription FactorsGene Expression Regulation, FungalMembrane Potential, MitochondrialMitochondriaOxidative PhosphorylationPromoter Regions, GeneticCCAAT-Binding FactorFep1 protein, S pombeGATA Transcription FactorsIronPhp4 protein, S pombeSchizosaccharomyces pombe ProteinsUbiquitin-Protein LigasesFep1Hul6Oxidative phosphorylationPhp4

Identifiers

PMID42489909

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.