Evidence map›Paper›PMID 42489485›Full record

ArticlemBio2026

AlphaFold-driven structural proteomics reveals extensive cellulosome machinery in human ruminococcal symbionts.

Christine Minor, Allen Takayesu, Mark A Arbing, Sung Min Ha, Robert P Gunsalus, Matteo Pellegrini, Michael R Sawaya, Robert T Clubb

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Christine Minor *Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.ORCID 0009-0005-5342-3917
Allen Takayesu *Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
Mark A ArbingUCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA.
Sung Min HaDepartment of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, California, USA.
Robert P GunsalusUCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA.ORCID 0000-0002-1937-8412
Matteo PellegriniUCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA.
Michael R SawayaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.ORCID 0000-0003-0874-9043
Robert T ClubbDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.ORCID 0000-0001-5718-3985

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI STEVEN E EALICK · 2018 to 2026
$34.2M
Research Training in Cell and Molecular BiologyT32GM145388 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Jorge Torres · 2022 to 2026
$5.2M
Molecular basis of heme scavenging by Gram-positive bacteriaR01AI161828 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2021 to 2026
$3.0M
Pixel Array Detector for Macromolecular CrystallographyS10OD021527 · OD · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2016 to 2016
$2.0M
HHS | National Institutes of Health (NIH) T32GM145388NIAID NIH HHS R01 AI161828NIGMS NIH HHS P30 GM124165NIGMS NIH HHS T32 GM145388NIH HHS R01-AI161828NIH HHS S10 OD021527U.S. Department of Energy (DOE) DE-FC02-02ER63421
6 · The paper itself

Abstract

Cellulosomes are large, surface-displayed enzyme complexes that enable anaerobic bacteria to degrade recalcitrant plant polysaccharides, yet cellulosome-expressing bacteria are thought to be rare in the human gut. Here, we show that extensive sequence divergence obscures the detection of many ruminococcal cellulosomes by conventional sequence homology-based methods. Using proteome-scale AlphaFold2 structural predictions, we uncovered a substantially expanded set of putative cellulosome-producing

Indexed as

Bacterial ProteinsCellulosomesCohesinsGastrointestinal MicrobiomeProteomicsRuminococcusAmino Acid SequenceCelluloseHumansPhylogenyProtein ConformationSymbiosisBacterial ProteinsCelluloseCohesinsAlphaFoldcellulosomehuman gutRuminococcusstructural proteomics

Identifiers

PMID42489485
PMCPMC13463774

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.