ArticleFrontiers in pharmacology2026
A modification of retrograde Langendorff-perfusion to increase the yield of Ca
Article in Frontiers in pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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10 authors.
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Abstract
Objective: Langendorff-perfusion is an established method to isolate cardiomyocytes from cardiac tissue for the investigation of cellular remodelling in cardiovascular diseases. Despite the existence of several protocols for isolation of murine atrial cardiomyocytes (aCM), extraction of a sufficient yield of Ca Methods: The effect of an additional ventricular ligature during Langendorff-perfusion on the yield of isolated aCM was evaluated in mice with cardiomyocyte-specific overexpression of CREM-IbΔC-X, a mouse model of pronounced atrial dilatation and spontaneous onset atrial fibrillation compared to littermate controls. Results: Atria with overexpression of CREM-IbΔC-X exhibited dilatation, increased mass and enhanced fibrosis. From such remodelled atria, we observed significantly lower extractions both of total and morphologically intact aCM compared to wildtype. The additional ventricular ligature did not affect total aCM yield in wildtype, but in fibrotic atria; the total aCM count was significantly increased (26 ± 4 vs. 15 ± 3 aCM/10 µL). Importantly, the number of morphologically intact, Ca Conclusion: The modification of an established Langendorff-perfusion protocol for aCM isolation by an additional ventricular ligature is associated with a numerically higher yield of morphologically intact, Ca
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