Evidence map›Paper›PMID 42488366›Full record

ArticleMultiple sclerosis journal - experimental, translational and clinical

Establishing demographic-based normative values for neurofilament light chain and glial fibrillary acidic protein in serum and plasma samples.

Tyler L Borko, Alanna Ritchie, Sean Selva, Stefan Sillau, Eric Engebretson, Andrew B Wolf, Enrique Alvarez, Robert Gross, Eric Gutierrez, John Corboy and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Multiple sclerosis journal - experimental, translational and clinical. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Tyler L BorkoDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0000-0002-6679-1867
Alanna RitchieDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Sean SelvaDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Stefan SillauDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Eric EngebretsonDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0009-0006-8875-5521
Andrew B WolfDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0000-0002-1406-4107
Enrique AlvarezDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Robert GrossDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Eric GutierrezDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0000-0002-9722-1699
John CorboyDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0000-0003-4169-1461
Diego SilvaBristol Myers Squibb, New York, NY, USA.
Ryan KammeyerDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.ORCID https://orcid.org/0000-0001-8657-3106
Amanda L PiquetDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Timothy L VollmerDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Salim ChahinDepartment of Neurology, Washington University in St. Louis, St. Louis, MO, USA.
Gregory OwensDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Jeffrey L BennettDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.
Kavita V NairDepartment of Neurology, University of Colorado Anschutz School of Medicine, Aurora, CO, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Interpretation of neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP) levels in blood requires reference ranges from healthy cohorts, accounting for demographic features. Objective: To compare serum and plasma NfL and GFAP in healthy individuals, accounting for age, sex, and body mass index (BMI). Methods: Adolescents through older adults were recruited at Colorado community events and a senior clinic. A neurologist reviewed self-reported medical histories. Paired serum and EDTA plasma samples were assayed using the Quanterix SR-X Simoa Neuro 4-Plex. Log-linear models estimated percent change per year of age, per BMI unit, and sex ratios. GFAP used a piecewise age model (knot at 30 years). Spearman correlation assessed serum-plasma concordance. Results: We enrolled 143 participants (ages = 16.4-85.9; 63.6% female; mean BMI = 26.7). Serum-plasma correlations were strong (NfL Conclusion: NfL and GFAP are strongly age-dependent and concordant across serum and plasma in healthy individuals. An online calculator provides age-, sex-, and BMI-adjusted reference estimates and serum/plasma conversions.

Indexed as

biomarkersdemyelinationGFAPMultiple sclerosisNfL

Identifiers

PMID42488366
PMCPMC13389189

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.