ArticleCureus2026
Identification of Plasmid-Mediated Colistin Resistance in Multidrug-Resistant Gram-Negative Rods Isolated From Immunocompromised Patients.
Article in Cureus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
5 authors.
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Abstract
introductionAntimicrobial resistance (AMR) poses a catastrophic threat to immunocompromised patients, particularly through the spread of multidrug-resistant (MDR) Gram-negative bacteria. With the rise of carbapenem resistance, colistin has returned as a last-resort therapy. However, the emergence of the mobilized colistin resistance (
methodsThis descriptive cross-sectional study was conducted over 12 months at Lahore General Hospital and the University of Health Sciences, Lahore. We collected samples of 100 MDR Gram-negative rods (GNRs) from biological specimens (urine, blood, pus, wound, tracheal secretions) of immunocompromised patients. Identification was confirmed via API 20E (Analytical Profile Index). Antibiotic susceptibility was determined using the Kirby-Bauer method. Crucially, colistin susceptibility was assessed using the CLSI-recommended colistin broth disk elution (CBDE) method. Polymerase chain reaction (PCR) was used to screen for
resultsThe sample comprised 100 isolates, predominantly
conclusionWhile the overall prevalence of colistin resistance (6%) is relatively low compared to carbapenems, the universal presence of
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