Evidence map›Paper›PMID 42487668›Full record

Observational studyJournal of medicine and life2026

Microbiota injury during conditioning for allogeneic hematopoietic stem cell transplantation.

Lavinia-Eugenia Lipan, Karina-Doris Vihta, Dumitru Jardan, Andi Palade, Iuliana Iordan, Alexandra Marcoci, Oana-Gabriela Craciun, Alina Daniela Tănase

Abstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Journal of medicine and life, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lavinia-Eugenia LipanCarol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Karina-Doris VihtaFundeni Clinical Institute, Bucharest, Romania.
Dumitru JardanMedLife Romania, Bucharest, Romania.
Andi PaladeMedLife Romania, Bucharest, Romania.
Iuliana IordanCarol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Alexandra MarcociCarol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Oana-Gabriela CraciunFundeni Clinical Institute, Bucharest, Romania.
Alina Daniela TănaseCarol Davila University of Medicine and Pharmacy, Bucharest, Romania.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a potentially curative treatment for hematological malignancies, but it is associated with substantial gut microbial dysbiosis linked to graft-versus-host disease, infections, and transplant-related mortality. The conditioning regimen represents the first major iatrogenic insult delivered within the transplant pathway, but its specific contribution to microbiota injury, independent of subsequent aplasia, antibiotic exposure, and mucositis, remains incompletely characterized. We conducted a prospective, single-center, observational longitudinal study at Fundeni Clinical Institute between August 2024 and February 2025, analyzing paired stool samples collected before conditioning (T0) and on the day of stem cell infusion (T1) from 47 adult allo-HSCT recipients. Microbial diversity was assessed using 16S rRNA gene sequencing (V4-V5 region), with the Shannon diversity index as the primary outcome. Statistical analyses were performed using SPSS, including Wilcoxon signed-rank tests for paired comparisons, Mann-Whitney U tests for between-group differences, and Kruskal-Wallis H tests. Shannon diversity decreased substantially between T0 and T1 (median 4.91 vs. 3.51; Wilcoxon

Indexed as

Hematopoietic Stem Cell TransplantationMicrobiotaTransplantation ConditioningAdultFemaleHumansLongitudinal StudiesMaleMiddle AgedProspective StudiesRNA, Ribosomal, 16STransplantation, HomologousRNA, Ribosomal, 16S16S rRNA sequencingaGVHD, acute graft-versus-host diseaseALL, acute lymphoblastic leukemiaallogeneic hematopoietic stem cell transplantationallo-HSCT, allogeneic hematopoietic stem cell transplantationAML, acute myeloid leukemiaASV, amplicon sequence variantCFA, cyclophosphamideCMBI, conditioning-associated microbiota injuryCNIs, calcineurin inhibitorsconditioning regimenCONSORT, Consolidated Standards of Reporting TrialsdysbiosisFLU, fludarabinegut microbiotaHAPLO, haploidentical donorIQR, interquartile rangeMAC, myeloablative conditioningMEL, melphalanMMF, mycophenolate mofetilMMUD, mismatched unrelated donorMRD, matched related donorMTX, methotrexateMUD, matched unrelated donorNMA, non-myeloablative conditioningPBSC, peripheral blood stem cellsPCoA, principal coordinates analysisPERMANOVA, permutational multivariate analysis of variancePTCy, post-transplant cyclophosphamideQ1–Q3, first to third quartileRIC, reduced-intensity conditioningrRNA, ribosomal ribonucleic acidShannon diversityT0, pre-conditioning timepoint (admission)T1, day of graft infusion (post-conditioning)TBI, total body irradiationtotal body irradiationTT, thiotepa

Identifiers

PMID42487668
PMCPMC13389782

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.