Evidence map›Paper›PMID 42487027›Full record

ArticleLab animal2026

Generation and characterization of humanized CD4 knock-in mice expressing chimeric mouse/human CD4 protein.

Ka-Man Kam, Tsz-En Shiu, Chien-Ming Hsieh, Wen-Ting Lu, Yu-Yun Pan, Chia-I Lin, Pin-Hung Lin, Ya-Hui Chuang, I-Shing Yu, Pei-Lung Chen

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Article in Lab animal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ka-Man KamGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.ORCID http://orcid.org/0009-0001-5122-371X
Tsz-En ShiuGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chien-Ming HsiehGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.
Wen-Ting LuGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.
Yu-Yun PanGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.ORCID http://orcid.org/0009-0008-1818-0456
Chia-I LinDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Pin-Hung LinGraduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Ya-Hui ChuangDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan. yahuichuang@ntu.edu.tw.ORCID http://orcid.org/0000-0003-0857-0035
I-Shing YuLaboratory Animal Center, College of Medicine, National Taiwan University, Taipei, Taiwan. isyu@ntu.edu.tw.ORCID http://orcid.org/0000-0002-2369-5816
Pei-Lung ChenGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan. paylong@ntu.edu.tw.ORCID http://orcid.org/0000-0002-5640-3074

Funding

National Taiwan University Hospital (NTUH) 112-S0309
6 · The paper itself

Abstract

Humanized mouse models have become indispensable tools for investigating human gene function and disease modeling. However, conventional transgenic approaches carry the risk of unforeseen biological consequences. Here, to address this concern, we developed a novel human CD4 knock-in (hCD4 KI) mouse model using CRISPR-Cas9 gene-editing technology. We replaced the region encoding the first two major extracellular domains of the mouse Cd4 gene, which are critical for interaction with major histocompatibility complex (MHC) class II, with the corresponding human CD4 sequence. Subsequently, we conducted comprehensive physiological and immune system analyses on hCD4 KI mice, including both heterozygous (CD4

Indexed as

CD4 AntigensGene Knock-In TechniquesAnimalsCD4-Positive T-LymphocytesCRISPR-Cas SystemsFemaleHumansMaleMiceMice, TransgenicCD4 Antigens

Identifiers

PMID42487027

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.