Evidence map›Paper›PMID 42483999›Full record

ArticleScience progress

Mitophagy suggesting a possible association with oocyte maturation: A study of granulosa cells from follicular fluid of normoresponder and diminished ovarian reserve cases.

Çağrı Öner, Damla Kolcuoğlu, Nergis Özlem Kiliç, Defne Dumanli, Ahmet Can Çelik, Alara Kaya, Baturalp Bakirci, Sena Köstü, Ece Bilge Şimşek, Belgin Selam and 1 more

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Article in Science progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Çağrı ÖnerMedical Faculty, Department of Medical Biology, Kırklareli University, Kırklareli, Turkey.
Damla KolcuoğluMedical Faculty, Department of Medical Biology, Kırklareli University, Kırklareli, Turkey.
Nergis Özlem KiliçMedical Faculty, Department of Histology and Embryology, Maltepe University, İstanbul, Turkey.ORCID 0009-0002-5710-257X
Defne DumanliMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Ahmet Can ÇelikMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Alara KayaMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Baturalp BakirciMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Sena KöstüMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Ece Bilge ŞimşekMedical Faculty, Kırklareli University, Kırklareli, Turkey.
Belgin SelamMedical Faculty, Department of Obstetrics and Gynecology, Acıbadem University, İstanbul, Turkey.
Ertuğrul ÇolakMedical Faculty, Department of Biostatistics, Eskişehir Osmangazi University, Eskişehir, Turkey.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ObjectiveThis study investigated mitophagy, a kind of mitochondrial dysfunction, as a biomarker for oocyte maturation by analyzing granulosa cells from follicular fluid in normoresponder (NOR) and diminished ovarian reserve (DOR) cases. The aim was to evaluate the expression of mitochondrial dynamics genes, Mitofusin-1 (MFN-1), Mitofusin-2 (MFN-2), and PARKIN, in granulosa cells surrounding oocytes at Metaphase I (MI) and Metaphase II (MII) stages.MethodsIn this experimental study, follicular fluid samples were collected from 30 NOR and 30 DOR women undergoing IVF and subdivided according to MI and MII oocyte maturation stages. Granulosa cells were isolated, morphologically assessed, and analyzed for gene expression using qRT-PCR.ResultsNOR MII granulosa cells exhibited the healthiest morphology and highest cell numbers, while DOR MI samples showed the most degeneration. MFN-1 expression was significantly decreased in DOR MI compared to NOR MI, while MFN-2 was higher in DOR MI and NOR MII. PARKIN expression was lower in DOR compared to NOR in both MI and MII. Correlation analysis revealed strong positive relationships among MFN-1, MFN-2, and PARKIN in NOR MII.ConclusionsThese findings suggest that mitophagy-related gene expression profiles in granulosa cells may serve as non-invasive biomarkers for predicting oocyte quality and embryo development in IVF.

Indexed as

Follicular FluidGranulosa CellsMitophagyOocytesOvarian ReserveAdultFemaleGTP PhosphohydrolasesHumansMitochondrial Membrane Transport ProteinsMitochondrial ProteinsUbiquitin-Protein LigasesGTP PhosphohydrolasesMfn1 protein, humanMFN2 protein, humanMitochondrial Membrane Transport ProteinsMitochondrial Proteinsparkin proteinUbiquitin-Protein Ligasesdiminished ovarian reservefollicular fluidMFN-1MFN-2normoresponderPARKIN

Identifiers

PMID42483999
PMCPMC13392319

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.