Integrated molecular analysis of granulosa cells and follicular fluid extracellular vesicles reveals predictive biomarkers of oocyte maturation in the southern white rhinoceros.

Ahmed Gad, Kristin Klohonatz, Nico G Menjivar, Dawit Tesfaye, Elena Ruggeri

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In one paragraph

Article in Frontiers in cell and developmental biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Ahmed Gad *Animal Reproduction and Biotechnology Laboratory (ARBL), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.

Kristin Klohonatz *Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.

Nico G MenjivarAnimal Reproduction and Biotechnology Laboratory (ARBL), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.

Dawit TesfayeAnimal Reproduction and Biotechnology Laboratory (ARBL), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.

Elena RuggeriReproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, Escondido, CA, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Assisted reproductive technologies (ARTs) are an imperative conservation tool to rescue species from extinction. An essential step towards technological advancements and ARTs' successes is identifying optimal Methods: This study aimed to identify transcriptomic differences in GC mRNA and miRNA expression, as well as miRNA cargo in FF-EVs in the southern white rhinoceros (SWR), and to integrate these datasets to uncover coordinated regulatory networks associated with oocytes that matured (with polarbody, PB) compared to those that failed to mature (without polar body, NoPB) following Results: Overall, in GCs, 1,117 transcripts (including both annotated and unannotated) were differentially expressed between groups. In GC-derived miRNAs, 11 were differentially expressed, all of which were upregulated in the PB group. In FF- EV samples, 15 miRNAs were identified as differentially expressed between the two groups. Comparison of predicted miRNA targets with the differentially expressed genes revealed multiple overlapping genes, suggesting a coordinated post-transcriptional regulation mechanism. Discussion: These miRNA-mRNA interactions are potential targets for further investigation, both as potential modulators to improve

Indexed as

assisted reproductive technologiesextracellular vesiclesmiRNAmRNAoocytesovum pickup

Identifiers

PMID42483213

DOI10.3389/fcell.2026.1849851

PMCPMC13385268

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.