Evidence map›Paper›PMID 42480515›Full record

ArticleThe American journal of tropical medicine and hygiene2026

Comparison of Oviposition and Adult Trapping to Monitor wMel Introgression for Wolbachia-Based Vector Control.

Elisabeth Nelson, Thiago N Pereira, Erica Milena de Castro Ribeiro, Bianca Daoud Mafra E Silva, Carolina Camillo, Thiago Rodrigues da Costa, Mauro M Teixeira, Albert I Ko, Derek A T Cummings, Luciano A Moreira

Abstract readComparative Study
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Elisabeth NelsonDepartment of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
Thiago N PereiraWorld Mosquito Program, Fundação Oswaldo Cruz Instituto Rene Rachou, Belo Horizonte, Brazil.
Erica Milena de Castro RibeiroWorld Mosquito Program, Fundação Oswaldo Cruz Instituto Rene Rachou, Belo Horizonte, Brazil.
Bianca Daoud Mafra E SilvaWorld Mosquito Program, Fundação Oswaldo Cruz Instituto Rene Rachou, Belo Horizonte, Brazil.
Carolina CamilloWorld Mosquito Program, Fundação Oswaldo Cruz Instituto Rene Rachou, Belo Horizonte, Brazil.
Thiago Rodrigues da CostaWorld Mosquito Program, Instituto Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Mauro M TeixeiraDepartment of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Albert I KoDepartment of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
Derek A T CummingsEmerging Pathogens Institute and Department of Biology, University of Florida, Gainesville, Florida, USA.
Luciano A MoreiraWorld Mosquito Program, Fundação Oswaldo Cruz Instituto Rene Rachou, Belo Horizonte, Brazil.

Funding

Optimizing Wolbachia-based interventions to ensure effective prevention of arboviral infectionsF31AI188834 · NIAID · YALE UNIVERSITY · PI Elisabeth Nelson · 2025 to 2026
$84k
NIAID NIH HHS F31 AI188834
6 · The paper itself

Abstract

Wolbachia introgression into Aedes aegypti (Ae. aegypti) mosquito populations is an effective strategy for preventing dengue and is currently under evaluation for WHO prequalification. The standard protocol for trials and deployments currently being conducted in Brazil by the World Mosquito Program (WMP) for long-term monitoring of Wolbachia introgression involves the use of BG-Sentinel traps (BG-traps; Biogents AG [Regensburg, Germany]), which are labor-intensive and costly. More affordable alternatives, such as oviposition traps (ovitraps), have been used in other settings; however, a comparison of the WMP protocol for Wolbachia surveillance has not been performed. As part of the EVITA dengue trial, Ae. aegypti eggs and adults were collected from 124 ovitraps and 237 BG-traps across 12 clusters in Belo Horizonte, Brazil, between March and May 2023. Eggs collected from ovitraps were reared to L3 and L4 larvae, and wMel Wolbachia was detected using quantitative polymerase chain reaction testing in larvae and adult mosquitoes from BG-traps. Mixed-effects models were used to compare cluster-level estimates of wMel introgression derived from ovitrap and BG-trap data over time. Among 3,675 larvae reared from ovitraps, wMel prevalence was 0.50 (95% CI: 0.48-0.51), whereas 1,244 adults from BG-traps had a prevalence of 0.45 (95% CI: 0.42-0.48). Cluster-level introgression estimates from larvae and adults were strongly correlated (Spearman's r = 0.70; P <0.001). Multivariate models incorporating current- and previous-month ovitrap data and mosquito abundance revealed strong agreement between ovitrap-based predictions and BG-trap estimates (rs ≥0.98). These findings reveal that ovitrap-based surveillance provides a low-resource, efficient, and precise alternative for monitoring Wolbachia introgression as interventions are scaled in high-burden dengue settings.

Indexed as

AedesMosquito ControlMosquito VectorsOvipositionPest Control, BiologicalWolbachiaAnimalsBrazilDengueFemaleLarva

Identifiers

PMID42480515
PMCPMC13539766

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.