Evidence map›Paper›PMID 42480009›Full record

ArticleIslets2026

Importance of antibody validation in detecting cell cycle regulatory protein p16

Jasmine Pipella, Peter J Thompson

Abstract readValidation Study
In one paragraph

Article in Islets, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jasmine PipellaDiabetes Research Envisioned and Accomplished in Manitoba (DREAM) theme, Children's Hospital Research Institute of Manitoba, Winnipeg, Canada.ORCID 0009-0005-9936-8386
Peter J ThompsonDiabetes Research Envisioned and Accomplished in Manitoba (DREAM) theme, Children's Hospital Research Institute of Manitoba, Winnipeg, Canada.ORCID 0000-0002-6851-8899

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

AIMS/

objectivep16

methodsFour commercial monoclonal antibodies reported to react against mouse p16

resultsOne commercial monoclonal antibody robustly detected a nuclear antigen in both mouse and human pancreas sections, consistent with the previously reported p16

conclusionOur findings highlight the importance of antibody validation for p16

Indexed as

Antibodies, MonoclonalCyclin-Dependent Kinase Inhibitor p16ImmunohistochemistryPancreasAnimalsHumansMiceMice, Inbred C57BLReproducibility of ResultsAntibodies, MonoclonalCyclin-Dependent Kinase Inhibitor p16antibody validationCell cycle regulationcyclin-dependent kinase inhibitorspancreatic beta cellssenescence

Identifiers

PMID42480009
PMCPMC13390536

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.