Protocol for quantifying Drosophila feeding behavior using flyPAD and optoPAD.

Nicholas J Collins, Madison N Endres, Irina T Sinakevitch, Lisha Shao

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Cellular source and circuit context organize functional specificity in thebioRxiv : the preprint server for biology · 2026
Article

4 · The record

Corrections and comments

Update of
Quantifying2026

5 · Who and what money

Authors and funding

4 authors.

Nicholas J CollinsDepartment of Biological Sciences, University of Delaware, Newark, DE, USA. Electronic address: nicollin@udel.edu.

Madison N EndresDepartment of Biological Sciences, University of Delaware, Newark, DE, USA.

Irina T SinakevitchDepartment of Biological Sciences, University of Delaware, Newark, DE, USA.

Lisha ShaoDepartment of Biological Sciences, University of Delaware, Newark, DE, USA. Electronic address: shaol@udel.edu.

Funding

Elucidating the molecular and neural structure of female sexual rewardR35GM147504 · NIGMS · UNIVERSITY OF DELAWARE · PI Lisha Shao · 2022 to 2026

$2.0M

NIGMS NIH HHS R35 GM147504

6 · The paper itself

Abstract

Quantifying feeding behavior with precision is essential for understanding how internal state, sensory cues, and neural activity influence food intake and dietary choice. Here, we present a protocol for performing consumption and dietary choice assays in Drosophila using the flyPAD/optoPAD system. We provide instructions for food preparation, arena setup, data acquisition, and analysis for studying feeding behavior, nutrient preference, and learning. This approach enables the measurement of feeding events across multiple arenas while allowing precise control of food substrates and optogenetic stimulation. For complete details on the use and execution of this protocol, please refer to Christie et al.

Indexed as

BehaviorHigh Throughput ScreeningModel Organisms

Identifiers

PMID42479491

DOI10.1016/j.xpro.2026.104711

PMCPMC13396711

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.