ArticleJournal of applied genetics2026
Impaired strigolactone biosynthesis and signalling are associated with promoter-associated CHH hypermethylation in Arabidopsis thaliana.
Article in Journal of applied genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Strigolactones are plant hormones that regulate diverse developmental processes and stress responses, but their potential role in epigenetic regulation remains poorly understood. Previous transcriptomic analyses of strigolactone mutants in various species revealed extensive transcriptional reprogramming, suggesting destabilisation of higher-order regulatory mechanisms rather than changes in discrete gene networks. Here, we investigated whether disruption in strigolactone biosynthesis or perception is associated with altered DNA methylation patterns. Whole-genome bisulfite sequencing was performed on 7-day-old seedlings of Arabidopsis thaliana wild-type and the strigolactone biosynthesis mutant max3 and the perception mutant d14. A comparative analysis identified differentially methylated regions in CG, CHG, and CHH sequence contexts. While CG methylation changes showed no directional bias, non-CG contexts exhibited a pronounced enrichment of hypermethylated regions in both mutants, with the strongest effect observed in the CHH context. CHH hypermethylation was preferentially enriched at promoter-associated regions, primarily overlapping non-coding and pseudogene loci and showed substantial overlap between max3 and d14, indicating a shared epigenetic response in both mutants. Gene-body-associated regions displayed a similar but weaker pattern. These results demonstrate that impairment of the strigolactone pathway is consistently associated with selective CHH hypermethylation rather than global methylation changes. Because CHH methylation in plants is commonly associated with RNA-directed DNA methylation, the observed pattern is consistent with altered RdDM-associated regulation; however, direct involvement of the RdDM machinery remains to be established. This study provides a genome-wide framework for exploring how strigolactones may be associated with epigenetic regulation in plants.
Indexed as
Identifiers
42479415What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.