Protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles in mouse testis.

Liying Wang, Huafang Wei, Yiyao Wu, Wei Li

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Liying WangGuangzhou Women and Children's Medical Center, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 510623, China.

Huafang WeiGuangzhou Women and Children's Medical Center, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 510623, China.

Yiyao WuGuangzhou Women and Children's Medical Center, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 510623, China.

Wei LiGuangzhou Women and Children's Medical Center, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 510623, China. Electronic address: leways@lzu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Male infertility affects 10%-20% of the population in most countries, with approximately half of the cases attributed to male factors. Here, we present a protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles (mRNA-LNPs) into the mouse testis. We describe steps for mRNA-LNP preparation, rete testis delivery, and assessment of transfection and spermatogenic rescue. This protocol establishes a relatively safe and efficient testis transfection system, offering a potential therapeutic strategy for patients with spermatogenic failure.

Indexed as

Cell BiologyDevelopmental biologyGenetics

Identifiers

PMID42475182

DOI10.1016/j.xpro.2026.104722

PMCPMC13393432

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.