Evidence map›Paper›PMID 42474357›Full record

ReviewJournal of clinical microbiology2026

The CDC/APHL next generation sequencing quality initiative: practical guidance to implementing quality management systems in laboratories performing next generation sequencing (phase 2).

Blake Cherney, Ariel Diaz, Christopher Ghattas, Mia Gregory, Emily Stow, Jennifer Onyewuenyi, Lakshmi Soujanya Kocherlakota, Lucy White, Eshaw Vidyaprakash, Sarah Scott and 6 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of clinical microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Blake CherneyOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.ORCID 0000-0002-1352-8539
Ariel DiazOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Christopher GhattasOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Mia GregoryOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Emily StowOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Jennifer OnyewuenyiOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Lakshmi Soujanya KocherlakotaOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Lucy WhiteOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Eshaw VidyaprakashOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Sarah ScottOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Jeffrey K LamarOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Glenita McKinneyOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Urmil ParekhOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Diego ArambulaOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.ORCID 0009-0005-5195-1871
Heather StangOffice of Laboratory Systems and Response, Division of Laboratory Systems, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.ORCID 0000-0002-5596-4793
CDC/APHL NGS Quality Initiative

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Whenever next generation sequencing is used in clinical and public health laboratories, rigorous quality control and quality assurance are key components of a quality management system. However, both the development and maintenance of a quality management system encompassing next generation sequencing present significant challenges, such as selection of appropriate sequencing platforms, complex workflows, allocation of time and resources, kit and reagent management, potential discontinuation of technical support, specialized bioinformatic workflows, compliance with regulatory and/or accreditation bodies, and the necessity of highly trained personnel. In an effort to address such challenges, the Centers for Disease Control and Prevention collaborated with public health partners to establish the Next Generation Sequencing Quality Initiative, which focuses on the development of a sequencing-focused quality management system. Here, we describe the ongoing efforts of the Next Generation Sequencing Quality Initiative to develop a foundational quality management system specific to next generation sequencing, as well as collaborations aimed to assist partners in addressing ongoing and emerging quality-related challenges associated with assay implementation in laboratories for surveillance and diagnostic applications.

Indexed as

High-Throughput Nucleotide SequencingLaboratoriesLaboratories, ClinicalQuality Assurance, Health CareCenters for Disease Control and Prevention, U.S.HumansQuality ControlUnited StatesClinical and Laboratory Standards Instituteclinical laboratorymethod validationnext generation sequencingpublic health laboratoriesquality assurancequality controlquality management systemQuality System Essentialsregulatory

Identifiers

PMID42474357
PMCPMC13456147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.