ArticleVirus genes2026
Molecular characterization and recent spread of dengue virus type 3 lineage III_B.3.2 in Brazil: in silico analysis of protein motifs and epidemiology.
Article in Virus genes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dengue virus (DENV) belongs to the Flaviviridae family and is responsible for a febrile arbovirus disease of global relevance. In Brazil, there has been endemic circulation of the four viral serotypes since the last century, with DENV-3 being frequently associated with more severe clinical conditions and major epidemics. This study aimed to perform the motif characterization of DENV-3 strains circulating in Brazil and its epidemiology. We obtained cases information by serotype from DATASUS/TABNET from 2013 to 2025. Furthermore, 184 complete genomes of Brazilian samples available in the GenBank and GISAID databases were analyzed with the genome reference sequence of DENV-3 (NC_001475.2). The sequences were edited and analyzed with the MAFFT, BioEdit, Nextclade, and GeneDoc software to identify lineages and amino acid motifs and were subsequently submitted to phylogenetic analyses in the IQ-Tree and FigTree programs. The results indicated a historical predominance of serotype one, but with a considerable increase in DENV-2 and DENV-3 cases in 2024 compared to the previous year. By December 2025, DENV-3 cases had already surpassed those of DENV-1 for that year. Nine amino acid motifs were identified throughout the genome, especially sites of N-myristoylation, casein kinase II phosphorylation, protein kinase C phosphorylation, and N-glycosylation. The predominant lineages were 3III_C.2 and 3III_C.2.2 until 2022, when lineage 3III_B.3.2 was introduced in Brazil, being associated with the increase in cases. This genotype presented substitutions and the absence of protein motifs compared to previous lineages, suggesting that such alterations contributed to its replicative success.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.