Evidence map›Paper›PMID 42472687›Full record

ArticleChemMedChem2026

A Unified Strategy Based on laeA Gene Introduction and Synthetic Approaches for Rediscovery of 3-Chlorogentisyl Alcohol as an Antibacterial Compound Against Acinetobacter baumannii.

Sota Honma, Taichi Kamo, Aoi Kimishima, Paul Wasuwanich, Hidehito Matsui, Yukiko Ujie, Masako Honsho, Hiroki Kojima, Toshiyuki Tokiwa, Akihiro Sugawara and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemMedChem, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Sota HonmaGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Taichi KamoGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.ORCID https://orcid.org/0009-0003-7166-0743
Aoi KimishimaGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.ORCID https://orcid.org/0000-0002-2625-1356
Paul WasuwanichDepartment of Internal Medicine, Mount Carmel Health System, Grove City, Ohio, USA.
Hidehito MatsuiGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Yukiko UjieGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Masako HonshoGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Hiroki KojimaGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Toshiyuki TokiwaGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
Akihiro SugawaraGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.ORCID https://orcid.org/0000-0003-1266-7497
Yasuko ArakiResearch and Development Division, Kikkoman Corporation, Noda-shi, Chiba, Japan.
Tadashi TakahashiResearch and Development Division, Kikkoman Corporation, Noda-shi, Chiba, Japan.
Kotaro ItoResearch and Development Division, Kikkoman Corporation, Noda-shi, Chiba, Japan.
Yukihiro AsamiGraduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.ORCID https://orcid.org/0000-0003-1821-9169

Funding

Japan Agency for Medical Research & Development (AMED) JP21am0101096Japan Society for the Promotion of Science 25K18642Platform Project for Supporting Drug Discovery and Life Science Research
6 · The paper itself

Abstract

Acinetobacter baumannii is a Gram-negative pathogen which is widespread in nature. Although antibiotics such as carbapenems and polymyxins are used to treat A. baumannii infections, the emergence of multidrug-resistant A. baumannii has greatly limited treatment options. Thus, A. baumannii infections are regarded as a great threat to global health. Previously, we successfully introduced the laeA gene into fungal strains in order to significantly increase the production of secondary metabolites, allowing us to create an original library (the ŌmuraLaeA-Introduced Fungal (ŌLF) library) and discover novel biological activities. In this study, we aimed to discover antibacterial compounds against A. baumannii from the ŌLF library. Following the screening program, 3-chlorogentisyl alcohol (1) was isolated from the Penicillium concentricum KTF-0317 strain, which produced the compound in higher amounts than the corresponding wild-type strain. Our investigations found that 1 demonstrated strong antibacterial activity even against quinolone - resistant A. baumannii strain. However, difficulties were encountered in the purification of natural compound 1. Therefore, synthetic samples were prepared via total synthesis and supplied for biological evaluation, enabling clarification of the structure-activity relationships. Our innovative approach revealed the overlooked biological activity of 1 and showed the prolificity and usefulness of the ŌLF library.

Indexed as

Acinetobacter baumanniiAnti-Bacterial AgentsDose-Response Relationship, DrugMicrobial Sensitivity TestsMolecular StructurePenicilliumStructure-Activity RelationshipAnti-Bacterial Agentsanti‐Acinetobacter baumannii candidateslaeAnatural productsPenicillium sp.structure–activity relationships

Identifiers

PMID42472687
PMCPMC13381315

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.