Evidence map›Paper›PMID 42470640›Full record

ArticleSTAR protocols2026

Isotope-free mapping of protein-RNA interactions at single-nucleotide resolution by iCLIP3.

Vladimir Despic, Melina Klostermann, Anna Orekhova, Mikhail Mesitov, Anke Busch, Kathi Zarnack, Julian König, Michaela Müller-McNicoll

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Vladimir DespicInstitute of Molecular Biosciences, Goethe University Frankfurt, 60438 Frankfurt Am Main, Germany; Cluster of Excellence SubCellular Architecture of Life (SCALE), Goethe University Frankfurt, 60438 Frankfurt am Main, Germany. Electronic address: despic@bio.uni-frankfurt.de.
Melina KlostermannTheodor Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany.
Anna OrekhovaTheodor Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany.
Mikhail MesitovTheodor Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany.
Anke BuschInstitute of Molecular Biology (IMB), 55128 Mainz, Germany.
Kathi ZarnackTheodor Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany; Cluster for Nucleic Acid Sciences and Technologies - NUCLEATE, 81377 Munich, Germany.
Julian KönigTheodor Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany; Cluster for Nucleic Acid Sciences and Technologies - NUCLEATE, 81377 Munich, Germany. Electronic address: julian.koenig@uni-wuerzburg.de.
Michaela Müller-McNicollInstitute of Molecular Biosciences, Goethe University Frankfurt, 60438 Frankfurt Am Main, Germany; Cluster of Excellence SubCellular Architecture of Life (SCALE), Goethe University Frankfurt, 60438 Frankfurt am Main, Germany; Max Planck Institute of Biophysics, 60438 Frankfurt Am Main, Germany. Electronic address: mueller-mcnicoll@bio.uni-frankfurt.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) enables transcriptome-wide mapping of RNA-binding protein (RBP)-RNA interactions. Here, we present iCLIP version 3 (iCLIP3), a streamlined protocol optimized for generating high-quality iCLIP libraries from low-input material. We describe steps for near-infrared visualization of RBP-RNA complexes, silica column-based RNA isolation, and unique dual indexing using TruSeq adapters for cost-effective multiplexing and sequencing. We detail a complete bioinformatics workflow to identify crosslinking events and define RBP binding sites from raw sequencing data.

Indexed as

Gene ExpressionRNAseqSequence analysis

Identifiers

PMID42470640
PMCPMC13400664

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.