ArticleBiotechnology letters2026
Enhanced biodiesel production from Fusarium oxysporum via mutagenesis and process optimization using bagasse hydrolysate as a sustainable carbon source.
Article in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiodiesel offers a sustainable alternative to fossil fuels with reduced greenhouse gas emissions; however, its commercial viability is constrained by high production costs. This study aimed to develop a cost-effective strategy for enhanced lipid and biodiesel production using Fusarium oxysporum NRC 2017, cultivated on sugarcane bagasse hydrolysate enzymatically saccharified by Bacillus cereus 3SME, as an inexpensive carbon source.
methodsTo improve lipid accumulation, the fungal strain was subjected to chemical and physical mutagenesis using gamma radiation (Ɣ-rays), ethidium bromide (EtBr), ethyl methane sulfonate (EMS), and sodium azide (NaN₃). Genetic variation between the wild type and induced mutants was confirmed by inter-simple sequence repeat (ISSR) analysis. The highest lipid-producing mutant, F. oxysporum NRC 2017-1, derived from gamma radiation, was selected for further optimization. Critical process parameters were optimized using response surface methodology (RSM) to maximize lipid yield.
resultsUnder optimized conditions, the mutant strain achieved a maximum lipid yield of 3.81 g/L, representing a substantial increase over the wild type. Gas chromatography analysis revealed a favorable fatty acid methyl ester (FAME) profile, dominated by C16-C18 fatty acids, which meets the key compositional requirements for high-quality biodiesel. Furthermore, the physicochemical properties of the produced biodiesel, including cetane number, viscosity, and density, were within the recommended limits of international standards (ASTM D6751 and EN 14214).
conclusionThis study presents an integrated and sustainable approach combining strain improvement via mutagenesis, valorization of lignocellulosic agricultural waste, and statistical process optimization. The findings highlight the potential of F. oxysporum NRC 2017-1 as a promising candidate for scalable, cost-effective biodiesel production.
Indexed as
Identifiers
42470572What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.