Evidence map›Paper›PMID 42470563›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

In Vivo Rice Haploid-Induction System by an Egg Cell-Specific Peptidase Knockout.

Ce Shi, Xuecheng Zhang, Zifu Zhao, Meng-Xiang Sun

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ce ShiState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
Xuecheng ZhangState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
Zifu ZhaoState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
Meng-Xiang SunState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China. mxsun@whu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Doubled haploid (DH) technology is a fast and convenient approach for crop breeding and genetic research. Currently, in vitro anther culture is the main method for rice haploid production. However, genotype dependence remains a major problem in the anther culture of most rice subspecies or cultivars for haploid induction. In this chapter, we describe a protocol for in vivo haploid induction in rice using egg cell-specific peptidase (ECS) knockout lines, including mutation of ECS using CRISPR-Cas9 system, selection of homozygous Osecs mutants, and identification of haploids in the offspring of Osecs by flow cytometry. The ECS mediated maternal in vivo haploid-induction system is a convenient, time-saving and labor-saving technique to produce rice DH lines.

Indexed as

Gene Knockout TechniquesHaploidyOryzaPeptide HydrolasesPlant ProteinsCRISPR-Cas SystemsPlant BreedingPlants, Genetically ModifiedPeptide HydrolasesPlant ProteinsAspartic proteasesEgg cellHaploid-inductionRice

Identifiers

PMID42470563

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.