Evidence map›Paper›PMID 42469942›Full record

ArticleBiological research2026

Mycophenolate mofetil induces angiogenesis disorders in zebrafish embryos by inhibiting the mitogen-activated protein kinase signaling pathway.

Mengqi Wan, Zhonghao Xiao, Xiaowen Shi, Zhijun Ye, Jiejun Liu, Xuyang Liu, Huimin Li, Xinjun Liao, Shouhua Zhang, Juhua Xiao and 1 more

Abstract read
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In one paragraph

Article in Biological research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mengqi Wan *Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Zhonghao Xiao *Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Xiaowen Shi *Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Zhijun YeJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Jiejun LiuJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Xuyang LiuKey Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Huimin LiJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Xinjun LiaoJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China.
Shouhua ZhangDepartment of General Surgery, Jiangxi Provincial Children's Hospital, The Affiliated Children's Hospital of Nanchang Medical College, Nanchang, Jiangxi, China.
Juhua XiaoDepartment of Ultrasound, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, 330006, Jiangxi, China.
Zigang CaoJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, College of Life Sciences, Clinical Research Center of Jinggangshan University, Jinggangshan University, Ji'an, 343009, China. zigangcao@126.com.ORCID http://orcid.org/0000-0002-8463-4996

Funding

iangxi Province Key Laboratory of Child Development 2024SSY06191Major Discipline Academic and Technical Leaders Training Program of Jiangxi Province 20204BCJL23043National Natural Science Foundation of China 32370896National Natural Science Foundation of China 82260307Science Fund for Distinguished Young Scholars of Jiangxi Province 20224ACB215001Science Technology Foundation of Jiangxi Province 20242BAB25444
6 · The paper itself

Abstract

As a commonly used immunosuppressant, mycophenolate mofetil (MMF) is widely prescribed after organ transplantation because it is not toxic to the liver and kidney. However, its use is associated with certain gastrointestinal toxicity and increased risk of opportunistic infections. Meanwhile, whether it affects vascular development is unclear. Herein, a zebrafish model was utilized to evaluate whether MMF affects vascular development, and the results showed that MMF exhibited teratogenicity, shortened the body length, caused pericardial edema, and delayed yolk sac absorption in zebrafish. In addition, although Intersegmental vessels showed hyperbranching, opposite changes were observed in cerebrovascular, with cerebrovascular inhibition. Moreover, MMF exposure upregulated oxidative stress levels, inhibited notch signaling and mitogen-activated protein kinase (MAPK) signaling, and affected the expression of vascular endothelial growth factor and receptor tyrosine kinase. Treatment with the antioxidant astaxanthin and the notch signaling pathway activator sodium valproate did not rescue the vascular development defects, indicating that MMF affects multiple signal pathways. However, the treatment of MMF-exposed zebrafish with guanosine and the MAPK signaling activators diprovocim significantly rescued the angiogenesis-related defects, suggesting that MMF induces ISV hyperbranching in zebrafish by inhibiting MAPK signaling and inosine 5'-monophosphate dehydrogenase (IMPDH).

Indexed as

AngiogenesisEmbryo, NonmammalianImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMycophenolic AcidNeovascularization, PathologicZebrafishAnimalsOxidative StressSignal TransductionImmunosuppressive AgentsMitogen-Activated Protein KinasesMycophenolic AcidMAPK signalingMycophenolate mofetilVascular developmentZebrafish

Identifiers

PMID42469942
PMCPMC13663165

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.