ArticleAdvanced biosystems2018
Engineering Neural Tissue from Human Pluripotent Stem Cells Using Novel Small Molecule Releasing Microspheres.
Article in Advanced biosystems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed.
- 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.Advanced healthcare materials · 2025Article
- Microspheres for 3D bioprinting: a review of fabrication methods and applications.Frontiers in bioengineering and biotechnology · 2025Review
- 3D bioprinting patient-derived induced pluripotent stem cell models of Alzheimer's disease using a smart bioink.Bioelectronic medicine · 2023Article
- 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink.Biomolecules · 2021Article
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues.Bioengineering (Basel, Switzerland) · 2021Review
- Tooth Formation: Are the Hardest Tissues of Human Body Hard to Regenerate?International journal of molecular sciences · 2020Review
- 3D Bioprinting Pluripotent Stem Cell Derived Neural Tissues Using a Novel Fibrin Bioink Containing Drug Releasing Microspheres.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here a novel technique for engineering neural tissue consisting of motor neurons by combining human-induced pluripotent stem cells (hiPSCs) with small molecules releasing microspheres is demonstrated. First, the small molecule purmorphamine (puro) is successfully encapsulated into poly ε-caprolactone (PCL) microspheres using a single emulsion oil-in-water (o/w) method for the first time with an efficiency of (84% ± 2.12%). These microspheres release 91% ± 1.7% of the encapsulated puro in a controlled fashion over 46 days. Puro microspheres, along with previously characterized retinoic acid (RA) releasing microspheres, are then incorporated into hiPSC aggregates to engineer neural tissue. The combination of puro and RA microspheres promotes hiPSC differentiation as indicated by the expression of multiple neural markers, including the neuronal marker β-tubulin III (βT-III), and the transcription factor Olig2 (7.69 ± 8.38%) on day 28. These tissues express the motor neuron marker HB9 (24.85 ± 4.51%) on day 35, and the mature motor neuron marker ChaT (12.35 ± 4.17%) on day 60. These engineered tissues can be used for regenerative medicine applications such as treating spinal cord injury (SCI), disease modeling, and drug screening.
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Registered trials
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