Evidence map›Paper›PMID 42464548›Full record

ArticleZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery2026

[Research on ferroptosis induced by RSL3, Erastin, and TBHP in rat intervertebral disc annulus fibrosus cells].

Xiaolin Pan, Jing Tan, Yuying Liu, Guihua Lei, Fengjuan Lü

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Xiaolin PanSCUT-UWA Joint Research Centre for Regenerative Medicine, School of Medicine, South China University of Technology, Guangzhou Guangdong, 510006, P. R. China.
Jing TanSCUT-UWA Joint Research Centre for Regenerative Medicine, School of Medicine, South China University of Technology, Guangzhou Guangdong, 510006, P. R. China.
Yuying LiuSCUT-UWA Joint Research Centre for Regenerative Medicine, School of Medicine, South China University of Technology, Guangzhou Guangdong, 510006, P. R. China.
Guihua LeiSCUT-UWA Joint Research Centre for Regenerative Medicine, School of Medicine, South China University of Technology, Guangzhou Guangdong, 510006, P. R. China.
Fengjuan LüSCUT-UWA Joint Research Centre for Regenerative Medicine, School of Medicine, South China University of Technology, Guangzhou Guangdong, 510006, P. R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To explore the effects of RAS-selective lethal small molecule 3 (RSL3), Erastin, and tert-butyl hydroperoxide (TBHP) on ferroptosis of rat annulus fibrosus cells (AFCs), and to establish an effective Methods: AFCs were isolated and cultured from the caudal vertebrae of 6-week-old female Sprague-Dawley rats by enzymatic hydrolysis. The optimal treatment concentrations of RSL3, Erastin, and TBHP (used for establishing an Results: The optimal concentrations for treating rat AFCs were screened by CCK-8 method and rat AFCs were divided into control group, RSL3 (0.1 μmol/L) group, Erastin (0.5 μmol/L) group, and TBHP (50 μmol/L) group. Both RSL3 group and TBHP group downregulated the mRNA expression levels of GPX4, FTH1, SLC7A11, and PCNA, upregulated the mRNA expression levels of ACSL4 and PTGS2, decreased the protein expression levels of GPX4 and FTH1, and increased the protein expression level of PTGS2 ( Conclusion: RSL3 and TBHP are suitable for the establishment of ferroptosis models in rat AFCs, which is helpful for the subsequent study of ferroptosis in intervertebral disc degeneration.

Indexed as

Annulus FibrosusCarbolinesFerroptosisPiperazinestert-ButylhydroperoxideAnimalsCells, CulturedCyclooxygenase 2FemaleGlutathione PeroxidaseMalondialdehydeOxidative StressProliferating Cell Nuclear AntigenRatsRats, Sprague-DawleyReactive Oxygen SpeciesCarbolinesCyclooxygenase 2erastinGlutathione PeroxidaseMalondialdehydePiperazinesProliferating Cell Nuclear AntigenPtgs2 protein, ratReactive Oxygen SpeciesRNA, MessengerRSL3 compoundtert-Butylhydroperoxideannulus fibrosusErastinferroptosisIntervertebral disc degenerationmodelingRAS-selective lethal small molecule 3tert-butyl hydroperoxide

Identifiers

PMID42464548
PMCPMC13391939

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