Comparing Accuracy and Biases of DNA Metabarcoding, Hybridization Capture, and Metagenomic Sequencing for Quantifying Herbivore Diets.

Charlotte E Eriksson, Lisa Shipley, Darren A Clark, Taal Levi

Abstract readComparative StudyEvaluation Study

In one paragraph

Article in Molecular ecology resources, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Charlotte E ErikssonDepartment of Fisheries, Wildlife and Conservation Sciences, Oregon State University, Corvallis, Oregon, USA.ORCID https://orcid.org/0000-0003-3061-2318

Lisa ShipleySchool of the Environment, Washington State University, Pullman, Washington, USA.

Darren A ClarkOregon Department of Fish and Wildlife, La Grande, Oregon, USA.ORCID https://orcid.org/0000-0002-8738-9826

Taal LeviDepartment of Fisheries, Wildlife and Conservation Sciences, Oregon State University, Corvallis, Oregon, USA.

Funding

Federal Aid in Wildlife Restoration F23AF03162National Science Foundation 2317537USDA National Institute of Food and Agriculture, McIntire-Stennis Project WNP00848

6 · The paper itself

Abstract

DNA metabarcoding using relative read abundance (RRA) is commonly applied to estimate herbivore diet composition, yet its quantitative accuracy remains uncertain. We assessed taxonomic resolution and quantitative performance of RRA from DNA metabarcoding compared to metagenomic sequencing and hybridization capture, using deer scats from feeding trials and recreated diet samples using plant tissues. All methods recovered plant composition in recreated diets (R

Indexed as

DietDNA Barcoding, TaxonomicHerbivoryMetagenomicsNucleic Acid HybridizationAnimalsDeerPlantsSequence Analysis, DNAdiet analysisfeeding trialsherbivoresnext‐generation sequencingrelative read abundancetarget enrichment

Identifiers

PMID42464404

DOI10.1111/1755-0998.70175

PMCPMC13376231

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.