ArticleJournal of cannabis research2026
Development and validation of a low-cost spectrophotometric method for cannabidiol (CBD) quantification based on the Beam reaction.
Article in Journal of cannabis research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cannabidiol (CBD) is a compound of high pharmacological interest, requiring accessible analytical methods for its identification and measurement. This study proposes the transformation of the classic Beam reaction, traditionally a qualitative colorimetric test, into a robust, low-cost quantitative technique using UV-Vis spectrophotometry. The method is based on the formation of a specific chromophore between CBD/CBDA and a 5% ethanolic KOH solution. Spectral characterization identified a maximum absorbance peak at 530 nm. Kinetic analysis established that the reaction stabilizes after a 24-hour incubation period, ensuring high reproducibility for batch analysis. The optimized protocol demonstrated high linearity (R
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