Evidence map›Paper›PMID 42464304›Full record

ArticleMicrobial cell factories2026

Enhancing recombinant eGFP secretion in P. tricornutum by optimizing culture conditions.

Nesrine Gargouch, Aurélie Charrier, Charlotte Toustou, Gaël Bougaran, Bruno Saint-Jean, Muriel Bardor

Abstract read
In one paragraph

Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nesrine GargouchLaboratoire GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, Université de Rouen Normandie, Rouen, 76000, France. nesrine.gargouch@univ-rouen.fr.
Aurélie CharrierIFREMER, PHYTOX, Laboratoire PHYSALG, 44000, Nantes, France.
Charlotte ToustouLaboratoire GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, Université de Rouen Normandie, Rouen, 76000, France.
Gaël BougaranIFREMER, PHYTOX, Laboratoire PHYSALG, 44000, Nantes, France.
Bruno Saint-JeanIFREMER, PHYTOX, Laboratoire GENALG, 44000, Nantes, France.
Muriel BardorLaboratoire GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, Université de Rouen Normandie, Rouen, 76000, France.

Funding

Agence Nationale de la Recherche ANR-21-CE20-0038-001Région Normandie DURABILITESRégion Normandie OptimAlg
6 · The paper itself

Abstract

Phaeodactylum tricornutum is a promising candidate for the production of recombinant therapeutic proteins including antibodies. So far, low secretion yields remain a significant bottleneck for pharmaceutical applications. Towards the objective to increase production yield of secreted recombinant protein, this study investigates the optimization of environmental and nutritional parameters, under laboratory conditions, to maximize the production of a model secreted recombinant protein (eGFPsec) under the control of the Nitrate Reductase promoter (P

Indexed as

DiatomsGreen Fluorescent ProteinsRecombinant ProteinsCulture MediaHydrogen-Ion ConcentrationMicroalgaeNitratesPromoter Regions, GeneticSeawaterCulture Mediaenhanced green fluorescent proteinGreen Fluorescent ProteinsNitratesRecombinant ProteinsCulture mediumGreen fluorescent proteinpHPhaeodactylum tricornutumPPFD

Identifiers

PMID42464304
PMCPMC13656395

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.