Evidence map›Paper›PMID 42463561›Full record

ArticleInternational ophthalmology2026

Upregulation of S100A9 in orbital tissues of patients with thyroid eye disease.

Jianhao Cai, Suwen Zhao, Huijing Ye, Tsz Kin Ng, Yuhang Wang, Ling Li, Danping Huang, Zeyi Li, Yongjie Qin, Hongyang Zhang

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Article in International ophthalmology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Jianhao CaiDepartment of Ophthalmology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Dadao North Road, Guangzhou, 510000, Guangdong, China.
Suwen ZhaoDepartment of Ophthalmology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Dadao North Road, Guangzhou, 510000, Guangdong, China.
Huijing YeState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat-sen University, Guangzhou, China.
Tsz Kin NgJoint Shantou International Eye Centre of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.
Yuhang WangJoint Shantou International Eye Centre of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.
Ling LiDepartment of Ophthalmology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Dadao North Road, Guangzhou, 510000, Guangdong, China.
Danping HuangState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat-sen University, Guangzhou, China.
Zeyi LiJoint Shantou International Eye Centre of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.
Yongjie QinDepartment of Ophthalmology, Guangdong Eye Institute, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, China.
Hongyang ZhangDepartment of Ophthalmology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Dadao North Road, Guangzhou, 510000, Guangdong, China. hy3005716@163.com.

Funding

Chronic Disease Management Research Project of National Health Commission Capacity Building and Continuing Education Center GWJJMB202510025168Joint Shantou International Eye Centre of Shantou University and The Chinese University of Hong Kong Party Building Research Project Jsiecdj2026003Shantou Medical and Health Science and Technology Program 200629155261470
6 · The paper itself

Abstract

purposeTo identify the differentially expressed genes in the orbital tissues of patients with thyroid eye disease (TED).

methodsThe microarray datasets on the orbital tissues of TED patients were collected from the Gene Expression Omnibus DataSets. Upon filtering, the differentially expressed genes (DEGs) of the selected dataset were determined and evaluated by gene ontology analysis. The expressions of genes related to the TED pathophysiological changes were validated by SYBR Green polymerase chain reaction (PCR) and immunofluorescence analyses on separate TED and control orbital tissue specimens.

resultsAnalysis on the orbital tissue samples of TED patients in the GSE58331 dataset identified 74 DEGs. Gene ontology analysis revealed that the DEGs are involved in the endocytosis, innate immunity, and extracellular matrix. Out of the 10 selected genes, only S100A9 showed significant upregulation in the orbital tissues of TED patients as compared to that of the control subjects (P = 0.006) in the SYBR Green PCR validation experiment. Immunofluorescence analysis confirmed significantly elevated expression of S100A9 in the orbital tissues of TED patients (P = 0.008). The receiver-operating characteristic curve (ROC) analysis demonstrated that the area under the ROC curve of S100A9 was 0.79 for the discrimination of the TED patients from the control subjects.

conclusionThis study revealed the upregulation of S100A9 in the orbital tissues of TED patients, suggesting that it could be involved in the development of TED.

Indexed as

Calgranulin BGene Expression RegulationGraves OphthalmopathyOrbitUp-RegulationAdultFemaleGene Expression ProfilingHumansMaleMiddle AgedReal-Time Polymerase Chain ReactionROC CurveCalgranulin BS100A9 protein, humanDifferentially expressed genesGEO datasetsOrbital tissuesS100A9Thyroid eye disease

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PMID42463561

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