Evidence map›Paper›PMID 42462715›Full record

ArticleStem cell reports2026

Single cell CRISPR screen identifies antagonism between Nsd1-H3K36me2 and Ezh2-H3K27me3 orchestrates pluripotency transition.

Yuqing Zhu, Qingying Wang, Yanzi Cao, Linxiao Hou, Lin Yang, Chen Cheng, Shoudong Ye, Wei Li, Jin Zhang

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PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yuqing ZhuCenter for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University, Hefei 230601, China. Electronic address: zhuyuqing@ahu.edu.cn.
Qingying WangCenter for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University, Hefei 230601, China.
Yanzi CaoCenter for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University, Hefei 230601, China.
Linxiao HouCenter for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and Bone Marrow Transplantation Center of the First Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310058, China.
Lin YangDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5S 1A1, Canada.
Chen ChengCenter for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and Bone Marrow Transplantation Center of the First Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310058, China.
Shoudong YeCenter for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University, Hefei 230601, China. Electronic address: shdye@126.com.
Wei LiDepartment of Pharmacology and Physiology, University of Maryland School of Medicine, University of Maryland‑Institute for Health Computing, 6116 Executive Blvd, North Bethesda, MD 20852, USA. Electronic address: wli2@som.umaryland.edu.
Jin ZhangCenter for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and Bone Marrow Transplantation Center of the First Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: zhgene@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The transcriptional and epigenetic landscape imposes constraints on the self-renewal capacity and lineage specification potential of both naive and primed mouse embryonic stem cells (mESCs). CRISPR/Cas9-based functional screening coupled with single-cell RNA-seq (CROP-seq) establishes relationships between gRNA-mediated knockout genotype and transcriptome phenotype, providing a powerful tool to dissect gene regulatory networks. Here, we employed CROP-seq to investigate the epigenetic regulation governing the pluripotency network in mESCs. This highly sensitive method identified key genes essential for the acquisition and exit from pluripotency, and revealed a novel role for H3K36me2 in modulating DNA methylation through regulating the expression of Dnmt1 and Dnmt3a. Specifically, loss of Nsd1-mediated H3K36me2 delayed naive state exit, whereas Ezh2 deficiency accelerated primed entry. Collectively, our findings identify an epigenetic regulatory network critical for determining mESCs' pluripotent state transitions.

Indexed as

Enhancer of Zeste Homolog 2 ProteinHistone-Lysine N-MethyltransferaseHistonesIntracellular Signaling Peptides and ProteinsPluripotent Stem CellsSingle-Cell AnalysisAnimalsCell DifferentiationCRISPR-Cas SystemsDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenesis, GeneticMiceMouse Embryonic Stem CellsDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADnmt1 protein, mouseDnmt3a protein, mouseEnhancer of Zeste Homolog 2 ProteinEzh2 protein, mouseHistone-Lysine N-MethyltransferaseHistonesIntracellular Signaling Peptides and ProteinsCRISPR screenCROP-seqepigenetic regulationH3K36me2Nsd1

Identifiers

PMID42462715
PMCPMC13476881

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