Evidence map›Paper›PMID 42461727›Full record

ArticleSTAR protocols2026

Protocol for establishing rectal tumor organoid-derived monolayers in a microphysiological system to model radiotherapy.

Eloïse Bouges, Charlotte Segers, Marco Lezzerini, Natalie Leys, Sarah Lebeer, Jianbo Zhang, Felice Mastroleo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Eloïse BougesNuclear Medical Applications, Belgian Nuclear Research Center (SCK CEN), Boeretang 190, 2400 Mol, Belgium; Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address: eloise.bouges@sckcen.be.
Charlotte SegersNuclear Medical Applications, Belgian Nuclear Research Center (SCK CEN), Boeretang 190, 2400 Mol, Belgium.
Marco LezzeriniSwammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, the Netherlands.
Natalie LeysNuclear Medical Applications, Belgian Nuclear Research Center (SCK CEN), Boeretang 190, 2400 Mol, Belgium.
Sarah LebeerDepartment of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Jianbo ZhangSwammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, the Netherlands.
Felice MastroleoNuclear Medical Applications, Belgian Nuclear Research Center (SCK CEN), Boeretang 190, 2400 Mol, Belgium. Electronic address: felice.mastroleo@sckcen.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Patient-derived organoids offer greater human translational relevance than mouse models or immortalized cell lines, owing to their multicellular composition and patient-specific responses. Here, we present a protocol for establishing a human in vitro rectal tumor model for radiotherapy studies using patient-derived tumor organoids (PDTOs) and monolayer derivatives integrated into a microphysiological system (MPS). We describe steps for cultivating PDTOs, their transition into monolayers, and transfer into an MPS. This protocol enables radiotherapy modelling, toxicity testing, and adjuvant therapies screening.

Indexed as

Biotechnology and bioengineeringCancerMolecular BiologyOrganoids

Identifiers

PMID42461727
PMCPMC13382717

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.