ArticleSTAR protocols2026
Protocol for reconstructing 3D human skin equivalents on endogenous dermal matrix with optional pigmentation.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
4 authors.
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Abstract
Previous in vitro 3D skin models are limited by their reliance on animal-derived collagen, a poor representation of the human dermis, and low throughput. Here, we present a protocol for reconstructing 3D human skin equivalents on an endogenous dermal matrix, in 12- and 24-well format, with optional pigmentation. We describe the expansion and seeding of fibroblasts, keratinocytes, and melanocytes in non-contractile polystyrene scaffolds. We then detail air-lifting and epidermal maturation, resulting in a fibroblast-derived dermal matrix with a fully stratified epidermis. For complete details on the execution of this protocol, please refer to Tham et al.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.