Evidence map›Paper›PMID 42461543›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Stabilization of Enzymes via Covalent Tethering to Appropriately Tuned Random Copolymer Brushes.

Héctor Sánchez-Morán, Luciana Gonçalves, Daniel K Schwartz, Joel L Kaar

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Héctor Sánchez-MoránDepartment of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA.
Luciana GonçalvesDepartment of Chemical Engineering, Federal University of Ceará, Fortaleza, CE, Brazil.
Daniel K SchwartzDepartment of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA.
Joel L KaarDepartment of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA. joel.kaar@colorado.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immobilizing enzymes can often cause their inactivation due to enzyme-support interactions and changes in the enzyme's microenvironment. As such, the design of novel supports remains largely empirical, and a rational approach may avoid time-consuming and tedious experiments. This chapter describes a method for stabilizing enzymes via immobilization on heterogeneous polymer brush supports. By varying the composition of the brush, the enzyme-brush interface may be finely tuned to prevent enzyme unfolding and rescue denatured enzymes via a chaperone-like mechanism. The design of polymer brush supports with appropriately tuned compositions may be informed by the quantification of protein surface hydrophobicity using the hi-patch algorithm.

Indexed as

Enzymes, ImmobilizedPolymersEnzyme StabilityHydrophobic and Hydrophilic InteractionsLipasePolyethylene GlycolsEnzymes, ImmobilizedLipasePolyethylene GlycolsPolymersEnzyme stabilizationHeterogeneous supportLipasePoly(ethylene glycol)Polymer brushPoly(sulfobetaine)

Identifiers

PMID42461543

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.