ArticleFish physiology and biochemistry2026
Nigella sativa oil ameliorates malathion-ınduced toxicity in Nile tilapia: a multi-systemic and molecular evaluation.
Article in Fish physiology and biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- From traditional histopathology to virtual staining: deep learning approaches for assessing tissue lesions in farmed fish.Veterinary research communications · 2026Review
- Comparative effects of dietary inorganic and nano forms of selenium on growth, hematological, biochemical, and antioxidant responses in Nile tilapia (Oreochromis niloticus).Tropical animal health and production · 2026Article
- Nanotechnology-Based Immunostimulants in Aquaculture: Mechanisms, Advances and Practical Applications.Veterinary medicine and science · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigated the protective effects of dietary supplementation with 2% black cumin (Nigella sativa) oil (BCO) against malathion-induced toxicity in Nile tilapia (Oreochromis niloticus). Over a 72-day feeding trial, fish were divided into four groups: control, 2% BCO, malathion (LC₅₀/20), and malathion + 2% BCO. The results demonstrated that sublethal malathion exposure significantly impaired growth performance including final weight, weight gain, and specific growth rate and worsening the feed conversion ratio. Hematological analysis revealed a severe anemic response, marked by significant reductions in red blood cell (RBC) counts, hemoglobin (Hb), and hematocrit (Hct) levels. Furthermore, malathion induced profound hepatic and metabolic dysfunction, evidenced by elevated serum AST, ALT, ALP, glucose, and cholesterol levels. Oxidative stress was prominent in the malathion-exposed group, characterized by suppressed antioxidant enzyme activities (SOD, CAT, GPx) and a severe increase in lipid peroxidation (MDA). Molecular assessments of brain tissue showed a marked upregulation of pro-inflammatory (il-1β, tnf-α) and apoptotic (caspase-3) genes, indicating neuroinflammation. Histopathological examinations confirmed severe multi-organ damage, including branchial lamellar fusion, hepatic necrosis, renal tubular degeneration, and brain hyperemia. Conversely, dietary supplementation with 2% BCO significantly ameliorated these toxic effects. Notably, BCO supplementation effectively restored growth parameters and FCR to levels statistically comparable to the control group, normalized hematological and biochemical profiles, bolstered antioxidant defenses, and significantly downregulated neuroinflammatory and apoptotic gene expressions, thereby preserving overall tissue architecture. In conclusion, this study demonstrates that dietary Nigella sativa oil effectively mitigates malathion-induced toxic effects. Rather than presenting individual components as entirely novel, the true strength and novelty of this approach lie in the multi-systemic integration of tissue histopathology with underlying neuroinflammatory and apoptotic gene expressions in the brain, offering a comprehensive insight into the protective mechanisms of black cumin oil in aquaculture.
Indexed as
Identifiers
42461504What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.