Evidence map›Paper›PMID 42461472›Full record

ArticleMolecular biology reports2026

Novel SNPs in anti müllerian hormone gene related to post-partum anestrum in holstein friesian cattle.

Nessreen M M Gharieb, Ahmed S A Sosa, Al-H H El-Naby Al-Shimaa, Hassan R H Darwish, Mohamed M M El-Sokary, Mahmoud E A Abou El-Roos, Karima Gh M Mahmoud

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nessreen M M GhariebTheriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
Ahmed S A SosaAnimal Reproduction and Artificial Insemination Department, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Al-H H El-Naby Al-ShimaaTheriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
Hassan R H DarwishCell Biology Department, Institute of Biotechnology Research, National Research Centre, Dokki, 12622, Giza, Egypt.
Mohamed M M El-SokaryTheriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. Mohamed.alsokary@fvtm.bu.edu.eg.
Mahmoud E A Abou El-RoosTheriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
Karima Gh M MahmoudAnimal Reproduction and Artificial Insemination Department, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPostpartum anestrus (PPA) is a significant cause of infertility in dairy cattle, often delaying the resumption of ovarian cyclicity. Anti-Müllerian hormone (AMH) serves as a biomarker of endocrine activity and ovarian follicular reserve. This study investigated the association between AMH gene polymorphisms and prolonged PPA in Holstein-Friesian cattle. Methods and Results A total of 305 cows were categorized into three groups based on reproductive history of anestrum and rectal ultrasonography: cyclic, PPA 6-12 months, and PPA more than 12 months. Blood samples were collected in vacutainers coated with EDTA- and kept at -20 °C for subsequent genomic DNA isolation. Data were recorded for age at first calving (AFC), calving interval (CI), calf birth weight (CBW), milk yield (MY), and days in milk (DM) for all animals. Genotype and allele frequencies for Exon 1 and Exon 5 of the AMH gene were analyzed using PCR-SSCP. PCR amplification revealed bands of 343 bp for Exon 1 and 253 bp for Exon 5. Five distinct SSCP patterns were identified for Exon 1, while Exon 5 exhibited only two patterns. Sequence analysis confirmed five genotypes (GG, CC, AA, CA, and GT) in Exon 1, with three single-nucleotide polymorphisms (SNPs) identified: a synonymous mutation, rs462782396 (Chr7.21401756G > T), and two previously unreported SNPs, Chr7.21401691 C > A and Chr7.21401708 C > A. Exon 5 exhibited two genotypes (TT and CT) and harbored a missense mutation, rs43505518 (Chr7.21403549T > C). Conclusions Significant associations were observed between AMH gene polymorphisms and prolonged postpartum anestrus (PPA) in cattle, particularly with respect to AFC and DM.

Indexed as

AnestrusAnti-Mullerian HormoneAnimalsCattleExonsFemaleGene FrequencyGenotypePolymorphism, Single NucleotidePolymorphism, Single-Stranded ConformationalPostpartum PeriodAnti-Mullerian HormoneAMHCattlePolymorphismPPAReproductive and productive traits

Identifiers

PMID42461472

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.