Evidence map›Paper›PMID 42460335›Full record

ArticleBiomedical optics express2026

FLIM quantification of temperature-dependent enzyme-NADH binding dynamics.

Jocelyn Martinez, Anna Theodossiou, Linghao Hu, Alex J Walsh

Abstract read
In one paragraph

Article in Biomedical optics express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jocelyn MartinezDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, 77840, USA.ORCID https://orcid.org/0000-0002-6019-1625
Anna TheodossiouDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, 77840, USA.
Linghao HuDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, 77840, USA.
Alex J WalshDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, 77840, USA.ORCID https://orcid.org/0000-0003-3832-8207

Funding

Autofluorescence lifetime microscopy for label-free detection of cell metabolism for cell biology researchR35GM142990 · NIGMS · TEXAS ENGINEERING EXPERIMENT STATION · PI Alexandra Walsh · 2021 to 2026
$2.2M
NIGMS NIH HHS R35 GM142990
6 · The paper itself

Abstract

Metabolic enzyme-coenzyme interactions are temperature-dependent; yet, conventional biochemical assays lack the temporal and spatial resolution to resolve rapid thermal effects on cellular metabolism and cellular binding equilibria. Here, fluorescence lifetime imaging microscopy (FLIM) of the metabolic coenzyme nicotinamide adenine dinucleotide (NADH) was used to quantify steady-state and rapid temperature-dependent changes in enzyme-coenzyme binding dynamics through estimation of dissociation constants (

Identifiers

PMID42460335
PMCPMC13372349

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.