Evidence map›Paper›PMID 42459142›Full record

ArticleTransboundary and emerging diseases2026

Global Comparative Phylogeography of E. granulosus s.s. Inferred From Mitochondrial DNA: Integrated Datasets of GenBank and Newly Characterized Isolates From Afghanistan.

Tharheer Oluwashola Amuda, Sayed Ajmal Qurishi, Guo-Dong Dai, Yao-Dong Wu, Wei-Gang Chen, Li-Qun Wang, Mughees Aizaz Alvi, Awab Ghulam Rahim, Li Le, Nian-Zhang Zhang and 7 more

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Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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17 authors.

Tharheer Oluwashola AmudaState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.ORCID https://orcid.org/0000-0002-1987-0837
Sayed Ajmal QurishiState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Guo-Dong DaiState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.ORCID https://orcid.org/0000-0003-4706-1188
Yao-Dong WuState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Wei-Gang ChenState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Li-Qun WangState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Mughees Aizaz AlviDepartment of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan, uaf.edu.pk.ORCID https://orcid.org/0000-0002-8800-2953
Awab Ghulam RahimDepartment of Public Health, Faculty of Medical, Afghan International Islamic University, Kabul, Afghanistan.ORCID https://orcid.org/0000-0002-7380-1197
Li LeState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Nian-Zhang ZhangState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Wen-Hui LiState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Bao-Quan FuState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Hong YinState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Wan-Zhong JiaState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.
Xue-Nong LuoState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.ORCID https://orcid.org/0000-0001-7457-2325
Li LiState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.ORCID https://orcid.org/0000-0001-6467-0247
Hong-Bin YanState Key Laboratory of Animal Disease Control and Prevention/College of Veterinary Medicine, Lanzhou University/Gansu Province Research Center for Basic Disciplines of Pathogen Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs/National Para-reference Laboratory for Animal Echinococcosis/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China, caas.cn.ORCID https://orcid.org/0000-0002-8522-0875

Funding

Agricultural Science and Technology Innovation Program CAAS-ZDRW202607Gansu Province Joint Research Fund 24JRRA807Gansu Province Joint Research Fund 25JRRA1164Gansu Provincial Research-Industry Integration Science and Technology Empowerment Program 25FNNA002Innovation Program of Chinese Academy of Agricultural Sciences CAAS-ASTIP-2021-LVRIInnovation Program of Chinese Academy of Agricultural Sciences CAASTIP-2026-05Major Science and Technology Project of Gansu Province 22ZD6NA001Major Science and Technology Project of Gansu Province 23ZDNA007Major Science and Technology Project of Gansu Province 24ZD13NA008NBCITS CARS-37Science and Technology Projects of Rikaze Autonomous Region, China RKZ2025ZD-009
6 · The paper itself

Abstract

Cystic echinococcosis (CE), caused by Echinococcus granulosus sensu stricto (E. granulosus s.s.), remains a major zoonotic disease of global public health importance. Afghanistan is considered endemic for CE; however, molecular data on circulating parasite populations are extremely scarce. This study aimed to characterize the genetic diversity, population structure, and global phylogeographic relationships of E. granulosus s.s. from Afghanistan within an integrated worldwide mitochondrial dataset. Hydatid cysts were collected from livestock hosts (cattle, sheep, and goats) in Afghanistan, and mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes were sequenced and analyzed alongside a comprehensive global dataset retrieved from GenBank. Genetic diversity indices, neutrality tests, median-joining (MJ) haplotype (H) networks, sliding-window analyses, and phylogenetic reconstructions based on concatenated mitochondrial sequences were employed. All Afghan isolates were identified as E. granulosus s.s., with a strong predominance of genotype G1 and only a single G3 isolate. Afghan populations exhibited moderate to high H diversity (Hd) but low nucleotide diversity, indicating closely related Hs separated by few mutations. Global H networks revealed star-like topologies with extensive intercontinental H sharing and no host- or geography-specific clustering. Phylogenetic and network analyses confirmed the genetic coherence of the G1 lineage worldwide and the limited diversity of G3. Sliding-window analyses showed genome-wide neutrality and absence of localized selective sweeps. Collectively, these findings position Afghan E. granulosus s.s. populations within a globally interconnected transmission network, highlighting the role of livestock movement and historical dispersal in shaping contemporary CE epidemiology. This study fills a critical regional knowledge gap and provides a robust phylogeographic framework to inform surveillance, control, and One Health interventions in endemic settings.

Indexed as

DNA, MitochondrialEchinococcosisEchinococcus granulosusAfghanistanAnimalsGenetic VariationPhylogenyPhylogeographySheepSheep DiseasesDNA, MitochondrialAfghanistancystic echinococcosisE. granulosus s.s.livestock movementmitochondrial DNAphylogeographytransboundary transmission

Identifiers

PMID42459142
PMCPMC13373698

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