Evidence map›Paper›PMID 42458441›Full record

ArticleBMC veterinary research2026

Molecular epidemiological investigation of swine hepatitis e virus (sHEV) in Guangdong Province, China: prevalence, genotypic characteristics, and zoonotic risk.

Zhenwen He, Dingyu Liu, Qin Luo, Baoling Liu, Dongqi Chen, Hua Xiang, Xiaohu Wang, Gang Wang, Yuan Huang, Jing Chen and 1 more

Abstract read
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Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zhenwen He *Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Dingyu Liu *Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Qin LuoGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Baoling LiuGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Dongqi ChenDongguan Key Laboratory of Zoonosis, Dongguan Center for Animal Disease Prevention and Control, Dongguan, 523086, China.
Hua XiangGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Xiaohu WangGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Gang WangGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Yuan HuangGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Jing ChenGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Rujian CaiGuangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. cairujian@gdaas.cn.

Funding

Guangdong Province Key Laboratory of Livestock Disease Prevention 2023B1212060040Guangdong Provincial Cross-County Cluster Industrial Park for Live Pigs (Maoming) GDSCYY2022-002Guangdong Provincial Rural Science and Technology Specialists Key Assignment Project KTP20240183Modern Agricultural Research System Innovation Team Project of Guangdong Province 2024CXTD15 and 2024CXTD15-01the 2024 Annual Special Funds for Rural Revitalization Strategy-Innovation Team Construction Project for the Provincial Modern Agricultural Industry Technology Systemthe 2024CXTD22-05the Open Topic of the Key Laboratory of Zoonoses in Dongguan City RSS202502the Pig Intelligent Animal Disease Prevention and Treatment System K11-006the Science and Technology Plan Projects of Guangdong Province 2023B0202010014the Scientific and Technological Plan Projects of Guangzhou 2023B04J0137
6 · The paper itself

Abstract

backgroundHepatitis E virus (HEV) is a major zoonotic foodborne pathogen causing human acute hepatitis, with genotype 4 endemic in China and swine as its main reservoir. Guangdong has a large intensive swine industry, yet stratified molecular epidemiological data on swine HEV are lacking. This study explored the prevalence, risk factors and genetic traits of swine HEV in Guangdong to support zoonotic disease control.

methodsA total of 1280 pig fecal samples from farms and abattoirs were tested for HEV RNA via RT-nPCR targeting ORF2. All positive samples underwent Sanger sequencing, and partial isolates were amplified for near-full-length genomes. Phylogenetic trees were built using the maximum likelihood method with 1000 bootstraps. Samples were grouped by region, season and sampling site; subgroup detection rates were compared via χ² and Fisher's exact tests, and binary logistic regression identified independent risk factors (P < 0.05).

resultsThe overall HEV positive detection rate was 2.66% (34/1280, 95% CI: 1.85-3.69). Detection rates differed markedly by region (P < 0.001) and season (P < 0.05), with no discrepancy between farms and abattoirs (P > 0.05). Region and season were independent risk factors. All isolates were HEV genotype 4, dominated by subtype 4a plus minor 4b and 4d strains, with consistent genotyping results from ORF2 and full-length genomes. Wide genetic divergence existed among local strains, and amino acid mutations concentrated in the ORF1 hypervariable region.

conclusionSwine HEV prevalent in Guangdong Province mainly belongs to genotype 4 and exhibits obvious regional and seasonal distribution characteristics. Continuous and targeted surveillance in high-risk regions and peak epidemic seasons is recommended to reduce the zoonotic transmission risk of swine HEV and ensure public health safety.

Indexed as

Hepatitis EHepatitis E virusSwine DiseasesAnimalsChinaFecesGenotypeMolecular EpidemiologyPhylogenyPrevalenceRisk FactorsRNA, ViralSwineZoonosesRNA, Viralhepatitis E virusmolecular epidemiologyswinezoonotic transmission

Identifiers

PMID42458441

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.