Evidence map›Paper›PMID 42458256›Full record

ArticleMolecular medicine (Cambridge, Mass.)2026

ACL injury reprograms quadriceps myonuclear epigenetic and transcriptional signatures in mice.

Nicholas T Thomas, Camille R Brightwell, Thomas Carter, Alexander R Keeble, Sara Gonzalez-Velez, Christine M Latham, Allison M Owen, Yuan Wen, Kevin A Murach, Christopher S Fry

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Article in Molecular medicine (Cambridge, Mass.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nicholas T ThomasCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Camille R BrightwellCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Thomas CarterCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Alexander R KeebleCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Sara Gonzalez-VelezCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Christine M LathamCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Allison M OwenCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Yuan WenCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA.
Kevin A MurachExercise Science Research Center, Department of Health, University of Arkansas, Human Performance, and Recreation, Fayetteville, AR, USA.
Christopher S FryCenter for Muscle Biology, University of Kentucky, 900 S. Limestone Street, CTW 210A, Lexington, KY, 40536-0200, USA. cfr223@uky.edu.ORCID https://orcid.org/0000-0002-4207-6594

Funding

Myostatin Alters Muscle Composition as The Result of an ACL InjuryR01AR072061 · NIAMS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FRY, CHRISTOPHER · 2018 to 2023
$2.2M
Myonuclear Epigenetics of Skeletal Muscle Mass Regulation with AgeR00AG063994 · NIA · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI MURACH, KEVIN · 2021 to 2023
$735k
NIAMS NIH HHS R01AR072061NIA NIH HHS R00AG063994
6 · The paper itself

Abstract

backgroundACL injury is a debilitating sport-related injury that induces prolonged deficits in muscle size and strength. Using a time-course of in vivo fluorescent metabolic labeling of nascent RNA in an ACL injury mouse model, we identified upregulated myonuclear transcriptional output early following the injury.

methodsTo define altered myonuclear transcription, we bred HSA

resultsIntegration of these datasets revealed transcriptional reprogramming of quadriceps myonuclei following ACL injury toward upregulation of fibrosis related genes and downregulation of metabolism related genes. When integrating bulk tissue RRBS with bulk tissue RNAseq, we demonstrate a similar, but more robust pattern of transcriptional reprogramming, suggesting the altered epigenetic landscape following ACL injury extends beyond myonuclei.

conclusionsOur results support a model of epigenetically-encoded muscle maladaptation following orthopedic injury, with implications for understanding a "muscle memory" of persistent dysfunction.

Indexed as

Anterior Cruciate Ligament InjuriesEpigenesis, GeneticQuadriceps MuscleTranscriptomeAnimalsDisease Models, AnimalGene Expression ProfilingMaleMiceTranscription, GeneticExtracellular matrixInjuryMuscle memoryMyonucleiSkeletal muscle

Identifiers

PMID42458256

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.