LDH encapsulation and MpdsRNase disruption boost RNAi efficacy in Myzus persicae, with mRNA-miRNA profiles unraveling lethality by tandem dsRNA targeting MpEcR and MpdsRNase.

Li He, Yu Wang, Junhua Xie, Xiaoqin Cheng, Hongyu Zhang, Yanna Huang, Xueming Tang

Abstract read

In one paragraph

Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Li He *School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.ORCID https://orcid.org/0000-0001-8811-9898

Yu Wang *School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Junhua XieSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Xiaoqin ChengNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China-Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.ORCID https://orcid.org/0009-0002-3531-4467

Hongyu ZhangNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China-Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.ORCID https://orcid.org/0000-0002-6368-5903

Yanna HuangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Xueming TangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Funding

2025Yangtze River Delta Science and Technology Innovation Community Joint Research (Basic Research) Project 2025CSJZN01300National Key Research and Development Program of China 2023YFD1700700

6 · The paper itself

Abstract

backgroundThe globally significant pest Myzus persicae inflicts considerable economic damage via phloem-feeding behavior and plant virus transmission. Although RNA interference (RNAi) is a promising alternative control strategy, its effectiveness is compromised by double-stranded RNA (dsRNA) instability.

resultsSilencing MpdsRNase markedly improved dsRNA stability in the aphid hemolymph and elevated dsRNA-induced aphid mortality. Layered double hydroxide (LDH) nanoparticle encapsulation effectively protected dsRNA from nuclease-mediated degradation, and further enhanced aphid mortality caused by tandem-linked dsRNA targeting both MpEcR and MpdsRNase. Specifically, treatment with ds-(EcR + dsRNase) altered the expression profiles of numerous messenger RNAs (mRNAs) and microRNAs (miRNAs), including cuticle/lipid biosynthesis-related genes and miRNA animal-undef-429. Accordingly, administration of the tandem dsRNA construct targeting MpdsRNase and the down-regulated cuticle protein gene MpCP19 yielded an 82% mortality rate in M. persicae.

conclusionThese findings established a dual-strategy to improve RNAi efficiency for sustainable aphid control and offered novel targets for dsRNA-based aphid management. © 2026 Society of Chemical Industry.

Indexed as

AphidsInsect ControlInsect ProteinsRNA, Double-StrandedRNA InterferenceAnimalsMicroRNAsNanoparticlesRNA, MessengerInsect ProteinsMicroRNAsRNA, Double-StrandedRNA, MessengerdsRNasedsRNA stabilityexpression profileLDH nanoparticleMyzus persicaeRNAi efficiency

Identifiers

PMID42458226

DOI10.1002/ps.71070

PMCPMC13569233

What OpenQuestion holds

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Read underepoch 390

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None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.