Evidence map›Paper›PMID 42458093›Full record

ArticleAnalytical and bioanalytical chemistry2026

High-throughput monitoring of in vitro enzymatic reactions using probe electrospray ionization-mass spectrometry.

Nobuyuki Okahashi, Takeo Taniguchi, Takuma Suzuki, Takanari Hattori, Hidenori Takahashi, Kohsuke Honda, Fumio Matsuda

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nobuyuki OkahashiDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, The University of Osaka, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan. n-okahashi@ist.osaka-u.ac.jp.
Takeo TaniguchiDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, The University of Osaka, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Takuma SuzukiInternational Center for Biotechnology, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Takanari HattoriAnalytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo Kuwabara-Cho, Nakagyo-Ku, Kyoto, 604-8511, Japan.
Hidenori TakahashiAnalytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo Kuwabara-Cho, Nakagyo-Ku, Kyoto, 604-8511, Japan.
Kohsuke HondaIndustrial Biotechnology Initiative Division, Institute for Open and Transdisciplinary Research Initiatives, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Fumio MatsudaDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, The University of Osaka, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Funding

JST GteX JPMJGX23B4
6 · The paper itself

Abstract

Probe electrospray ionization-mass spectrometry (PESI-MS) is an ambient ionization technique that enables rapid and simple mass spectrometry with minimal sample preparation. In this study, we evaluated the applicability of PESI-MS for the quantitative analysis of metabolites in in vitro enzyme reaction mixtures, which often contain high concentrations of proteins, salts, and buffers that are not suitable for conventional MS. Using glycolytic metabolites as a model, we optimized the type of organic solvents for mixing, data processing method, and investigated the effects of additives and reaction components on ionization efficiency and signal stability. Ethanol and 2-propanol were identified as suitable solvents for the detection of sugar phosphates, organic acids, and nucleotides. The quantitative reproducibility was improved by employing a total peak-height-based data processing method. The presence of proteins had a minimal influence on ionization. Although high concentrations of MgCl

Indexed as

In vitro enzyme reactionNon-oxidative glycolysisPESI-MSProbe electrospray ionization

Identifiers

PMID42458093

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.