Evidence map›Paper›PMID 42457739›Full record

ArticleScientific reports2026

Use of organ transplant solution to preserve skeletal muscle for cellular and spatial transcriptomic analyses.

Prech Uapinyoying, Young Jae Moon, Aiping Zhang, Pamela Barraza-Flores, Won Lee, Angela N Viaene, Sanda Alexandrescu, Nhu Chau, Casie A Genetti, Yi-Wen Chen and 5 more

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Prech UapinyoyingNeuromuscular and Neurogenetic Disorders of Childhood Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, USA.
Young Jae MoonCenter for Precision Medicine and Genomics Research, Children's National Research Institute, Children's National Hospital, Washington, DC, 20012, USA.
Aiping ZhangCenter for Precision Medicine and Genomics Research, Children's National Research Institute, Children's National Hospital, Washington, DC, 20012, USA.
Pamela Barraza-FloresDivision of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Won LeeDivision of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Angela N ViaeneDepartment of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, Perelman School of Medicine, Philadelphia, USA.
Sanda AlexandrescuDepartment of Pathology, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Nhu ChauLaboratory Medicine and Pathology Research Core, Children's National Health System, Children's National Hospital, Washington, DC, 20010, USA.
Casie A GenettiDivision of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Yi-Wen ChenCenter for Precision Medicine and Genomics Research, Children's National Research Institute, Children's National Hospital, Washington, DC, 20012, USA.
Alan H BeggsDivision of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Carsten G BönnemannNeuromuscular and Neurogenetic Disorders of Childhood Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, USA.
Monkol LekDepartment of Genetics, Yale School of Medicine, New Haven, CT, USA.
Jyoti K JaiswalCenter for Precision Medicine and Genomics Research, Children's National Research Institute, Children's National Hospital, Washington, DC, 20012, USA. jkjaiswal@cnmc.org.
Pediatric Cell Atlas of SkeletalMuscle consortium

Funding

Neurobehavioral Evaluation CoreP50HD105328 · NICHD · CHILDREN'S RESEARCH INSTITUTE · PI WILLIAM Davis GAILLARD · 2021 to 2026
$9.3M
Chan Zuckerberg Initiative DAF2021-237666National Institute of Child Health and Human Development P50HD105328NICHD NIH HHS P50 HD105328
6 · The paper itself

Abstract

Single-cell and spatial transcriptomic technologies have revolutionized human cell and tissue research. Success of these methods critically depends on the preservation of RNA integrity, cellular viability, and structure of the explanted tissues-features that are impacted by tissue storage conditions, and pose challenges in research settings, where tissues are often collected in the operating room and transported to distant laboratories. In this study, we evaluated the effectiveness of a clinically approved organ transplant solution (TS) for preserving skeletal muscle under refrigerated conditions. Using murine and human skeletal muscle tissues we found that compared to buffered saline, TS consistently maintained higher RNA quality, preserved tissue morphology, and supported successful primary cell culture and spatial transcriptomic analysis of murine and human muscle samples obtained from different surgical collection sites. These findings establish a foundation for the use of a standardized protocol for live muscle tissue preservation for reliable downstream transcriptomic analyses for multicenter research efforts.

Identifiers

PMID42457739

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.