Evidence map›Paper›PMID 42455248›Full record

ArticleMolecular biology reports2026

Genetic diversity within Miscanthus × giganteus: Evidence from morphological traits and ITS rDNA in European accessions.

Hana Auer Malinská, Diana Polanská Nebeská, Michaela Kocholatá, Anna Erol, Josef Trögl

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Hana Auer MalinskáDepartment of Biology, Faculty of Science, Jan Evangelista Purkyne University in Usti nad Labem, Pasteurova 3632/15, Usti nad Labem, 40096, Czech Republic. hana.malinska@ujep.cz.ORCID http://orcid.org/0000-0001-7150-4044
Diana Polanská NebeskáDepartment of Biology, Faculty of Science, Jan Evangelista Purkyne University in Usti nad Labem, Pasteurova 3632/15, Usti nad Labem, 40096, Czech Republic.ORCID http://orcid.org/0000-0002-4388-5297
Michaela KocholatáCentre for Nanomaterials and Biotechnology, Faculty of Science, Jan Evangelista Purkyne University in Usti nad Labem, Pasteurova 3632/15, Usti nad Labem, 40096, Czech Republic.ORCID http://orcid.org/0000-0003-3321-0234
Anna ErolClinical Research Centre, Medical University of Bialystok, ul. Jana Kilińskiego 1, Bialystok, 15-089, Poland.ORCID http://orcid.org/0000-0002-9932-5176
Josef TröglDepartment of Environmental Chemistry and Technology, Faculty of Environment, Jan Evangelista Purkyne University in Usti nad Labem, Pasteurova 3632/15, Usti nad Labem, 40096, Czech Republic.ORCID http://orcid.org/0000-0003-0369-5082

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMiscanthus × giganteus (M × g) is a sterile allotriploid hybrid (M. sacchariflorus × M. sinensis) widely used for biomass production and generally considered genetically uniform due to clonal propagation and a strong founder effect. However, phenotypic variation observed in European-grown material suggests that this assumption may be oversimplified. METHODS AND

resultsThis study compared biometric, morphological, and genetic characteristics of four M × g accessions used for agronomy field trials in Croatia (HR), Ukraine (UA), Germany (DE), and the Czech Republic (CZ). Biometric analysis revealed that the HR accession produced 1.6-2-fold higher biomass yield, primarily associated with a 1.5-3-fold increase in sprout number. The HR accession also exhibited distinct morphological traits, including stem pigmentation and trichome presence. Sequencing of nuclear ribosomal DNA internal transcribed spacer markers (ITS1 and ITS2) identified seven polymorphic sites (four in ITS1 and three in ITS2) three of them unique to the HR accession, whereas UA, DE, and CZ displayed identical sequences. In silico comparison with reference sequences confirmed and further highlighted the marked divergence of the HR accession. Notably, variation at position 195 bp in ITS2 indicated fixation of a single ribotype in HR compared to heterozygosity in the remaining accessions.

conclusionsThe results suggest the presence of limited genetic variation within M × g, despite its predominantly clonal propagation. The observed association between ITS polymorphisms and phenotypic traits in the HR accession indicates that intra-specific variation may contribute to differences in biomass performance. These findings highlight the potential for identifying and utilizing divergent accessions in future breeding and selection programs, while also suggesting that the assumption of strict genetic uniformity in M × g may require reconsideration.

Indexed as

Genetic VariationPoaceaeDNA, PlantDNA, RibosomalDNA, Ribosomal SpacerEuropePhenotypePhylogenyPolymorphism, GeneticDNA, PlantDNA, RibosomalDNA, Ribosomal Spacerbiomassgenetic variabilityITS (internal transcribed spacer)Miscanthus × giganteusrDNA

Identifiers

PMID42455248
PMCPMC13372840

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