Evidence map›Paper›PMID 42455209›Full record

ArticleVeterinary research communications2026

Development and validation of an E2 based indirect ELISA for serological detection of porcine Getah virus.

Zijun Xiang, Wenjing Tian, Dong Li, Meiqi Liu, Zhicheng Han, Qiong Wu, Zhong Peng, Ling Zhao, Bin Wu, Danna Zhou

Abstract readValidation Study
PubMed Publisher
In one paragraph

Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zijun Xiang *National Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Wenjing Tian *National Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Dong LiWuhan Center for Animal Diseases Prevention and Control, Wuhan, 430000, China.
Meiqi LiuNational Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Zhicheng HanHubei Academy of Agricultural Sciences, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Pathogenic Microbiology of Hubei Province, Institute of Animal Sciences and Veterinary Medicine, Wuhan, 430064, China.
Qiong WuHubei Academy of Agricultural Sciences, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Pathogenic Microbiology of Hubei Province, Institute of Animal Sciences and Veterinary Medicine, Wuhan, 430064, China.
Zhong PengNational Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Ling ZhaoNational Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. zling604@outlook.com.
Bin WuNational Key Laboratory of Agricultural Microbiology; Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. wub@mail.hzau.edu.cn.
Danna ZhouHubei Academy of Agricultural Sciences, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Pathogenic Microbiology of Hubei Province, Institute of Animal Sciences and Veterinary Medicine, Wuhan, 430064, China. zdn66@hbaas.ac.cn.

Funding

National Key Research and Development Program of China 2024YFD1800500the National Natural Science Foundation of China No: 32503036
6 · The paper itself

Abstract

Getah virus (GETV) is an emerging mosquito borne alphavirus that has recently expanded in Chinese swine herds and causes reproductive losses and reduced growth in pigs. The absence of a standardized high throughput serological assay has limited surveillance and hindered accurate assessment of GETV circulation. In this study, we developed and validated an indirect ELISA based on recombinant full length E2 protein for detection of anti-GETV IgG in swine serum. The E2 gene from a circulating strain was expressed in Escherichia coli as inclusion bodies, followed by denaturation, redox refolding, and purification to > 90% purity. Positive and negative control sera were defined by virus neutralization. Systematic optimization identified 1 μg/mL antigen coating and 1:100 serum dilution as optimal, and the cutoff was set at OD

Indexed as

AlphavirusAlphavirus InfectionsSwine DiseasesViral Envelope ProteinsAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayRecombinant ProteinsSensitivity and SpecificitySwineAntibodies, ViralRecombinant ProteinsViral Envelope ProteinsDiagnostic sensitivity and specificityE2 proteinGetah virusIndirect ELISASerological surveillance

Identifiers

PMID42455209

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.