Transcriptomic Profiling of Canine Testicular Leydig Cell Tumors Uncovers Key Upregulated Gene Pathways.

Malgorzata Kotula-Balak, Recep Uyar, Emilia Morańska, Grzegorz Lonc, Ummu Gulsum Boztepe, Wojciech Lopuszynski

Abstract read

In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Malgorzata Kotula-BalakDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland.ORCID 0000-0002-6379-996X

Recep UyarGenetic Department, Institute of Forensic Science, Ankara University, Ankara 06520, Türkiye.

Emilia MorańskaDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.ORCID 0009-0000-4674-6375

Grzegorz LoncDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland.

Ummu Gulsum BoztepeDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland.ORCID 0000-0002-9277-6310

Wojciech LopuszynskiDepartment of Pathomorphology and Forensic Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland.ORCID 0000-0002-9875-5449

Funding

Narodowe Centrum Nauki OPUS26 2023/51/B/NZ4/00889

6 · The paper itself

Abstract

Total RNA was isolated from sections of healthy testes and Leydig cell tumors of mixed-breed dogs using TMA Master II device. The RNA-seq libraries were sequenced on the Illumina platform. Following differential expression analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were applied with quality control obtained using FastQC and Trimmomatic. This analysis revealed 1500 transcripts, including 928 upregulated and 168 downregulated genes. The results demonstrated that a significant proportion of these differentially expressed genes are directly involved in the control of sex steroid production (

Indexed as

angiogenesisdogestrogensLeydig cell tumorlipid homeostasisnext generation sequencingPI3K-Akt pathway

Identifiers

PMID42450712

DOI10.3390/ani16132005

PMCPMC13359613

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.