ArticleFoods (Basel, Switzerland)2026
The Hapten Design, Monoclonal Antibody Preparation, and Immunoassay Development for Rapid Detection of Isofenphos-Methyl.
Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
Isofenphos-methyl (IFP), a highly toxic and persistent organophosphate pesticide (OP), is widely used for soil pest control in crops but poses severe risks to ecological safety and human health because of its environmental accumulation and bioaccumulation. Herein, a sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for rapid IFP detection in vegetables. A novel IFP hapten was rationally designed and synthesized via computer-aided molecular simulation, and its structure was validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR). High-specificity anti-IFP monoclonal antibodies (mAbs) with strong anti-matrix interference were prepared for the first time using a matrix effect-enhanced screening strategy. The optimized ic-ELISA showed high sensitivity, with an IC
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