Layer-by-layer assembly of bioactive nanofilms for in situ gene transfection of a LAMA3 mutant on hydrogenated TiO₂ nanotubes: in vitro evaluation of epithelial adhesion and hemidesmosome formation.

Caiyun Wang, Ran Lu, Xu Cao, Yanting Mu, Su Chen

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Article in BMC oral health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Caiyun WangLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, International Medical Services and Healthcare Center, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100070, China.

Ran LuLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, International Medical Services and Healthcare Center, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100070, China.

Xu CaoLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, International Medical Services and Healthcare Center, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100070, China.

Yanting MuLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, International Medical Services and Healthcare Center, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100070, China.

Su ChenLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, International Medical Services and Healthcare Center, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100070, China. chensu@mail.ccmu.edu.cn.

Funding

BEIJING HOSPITALS AUTHORITY'S ASCENT PLAN DFL20221301BEIJING STOMATOLOGICAL HOSPITAL, CAPITAL MEDICAL UNIVERSITY YOUNG SCIENTIST PROGRAM YSP202108

6 · The paper itself

Abstract

backgroundReliable peri-implant epithelial sealing is paramount for the prevention of peri-implantitis. Remarkably, both hemidesmosomes (HDs) and the internal basal lamina, which notably includes laminin332-essential for transmucosal epithelial sealing-are significantly diminished compared to their natural tooth counterparts. In situ gene therapy has emerged as a promising approach for the spatial regulation of HD formation.

methodsIn this study, we aim to develop a bioactive coating with an exceptional capacity for the in situ transfection of a recombinant adenovirus carrying a mutant of the laminin α3 chain gene (Ad-mLAMA3), thereby improving transmucosal epithelial sealing. Dopamine (DA), graphene oxide (GO), and type Ⅳ collagen (COL-Ⅳ) multilayers-(DA/GO/COL-Ⅳ)10, designated DGC-were assembled layer by layer on hydrogenated TiO

resultsThe DGC nanofilms were successfully established on hydrogenated TiO

conclusionsOur findings demonstrate that H-DGC can be engineered as a bio-instructive and in situ interface, significantly enhancing epithelial cell adhesion and mature HD formation, and offering a strategy to prevent peri-implantitis.

Indexed as

HemidesmosomesLamininNanotubesTitaniumTransfectionAdenoviridaeCell AdhesionCoated Materials, BiocompatibleCollagen Type IVEpithelial CellsGraphiteHumansMutationPeri-ImplantitisCoated Materials, BiocompatibleCollagen Type IVgraphene oxideGraphiteLamininlaminin alpha 3Titaniumtitanium dioxideGene deliveryIn situ transfectionNanofilmsPeri-implantitisSoft tissue sealingTitanium implants

Identifiers

PMID42449345

DOI10.1186/s12903-026-09250-1

PMCPMC13660248

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.