Protocol for the generation of human medullary spinal trigeminal nucleus-like organoids and trigeminothalamic assembloids.

Wei Pang, Jinkui Zhu, Siyuan Chu, Yangfei Xiang

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Wei PangSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: pangwei1@shanghaitech.edu.cn.

Jinkui ZhuSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Siyuan ChuSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Yangfei XiangSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China. Electronic address: xiangyf@shanghaitech.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nucleus-specific brain organoids enable precise human brain research, yet refined hindbrain models are rare. Here, we present a guided differentiation protocol for generating human medullary spinal trigeminal nucleus-like organoids (hmSpVOs). We describe steps for constructing spinal trigeminal nucleus (SpV)-thalamus assembloids. Moreover, we outline the assays to characterize functional connections within these assembloids. For complete details on the use and execution of this protocol, please refer to Pang et al.

Indexed as

Cell cultureCell DifferentiationOrganoids

Identifiers

PMID42447000

DOI10.1016/j.xpro.2026.104705

PMCPMC13382238

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.