ArticleTropical animal health and production2026
Microscopy-PCR discordance and shifting epidemiology in South India: advancing diagnostic precision for control of morphologically similar bovine hemopathogens (Babesia, Theileria, Anaplasma) from a hospital-based study.
Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Advancing epidemiological insights is instrumental in optimizing region-specific hemopathogen control. A retrospective study in Chennai, South India screened 2,434 cattle over one year, revealing a 13.93% hospital-based prevalence of morphologically similar hemopathogen (Babesia, Theileria, Anaplasma) diseases (HPDs) by light microscopy (LM). Anaplasmosis predominated (8.63%), increasing during monsoon; theileriosis rose to 4.44%, babesiosis declined to 0.86%, with highest prevalence in multiparous Jersey crossbreds. Relative sensitivity, specificity, PPV and NPV of LM were prospectively estimated using PCR as an operational reference standard in cattle suspected of HPDs. Specific primers targeting Babesia (18 S rRNA, 450 bp), T. annulata (merozoite surface antigen, 721 bp) and A. marginale (major surface protein, 265 bp) were used. LM demonstrated a relative sensitivity of 73.08% and an NPV of 76.67%, reflecting under-detection of PCR-positives and false-negative classifications. Substantial LM-PCR agreement (κ = 0.72) with one-sided discordance supports a complementary LM-PCR diagnostic approach. Overlapping clinical profiles showed severe fever, lymph node, conjunctival and lacrimation changes in theileriosis, and jaundice in anaplasmosis; infected cattle had lymphocytosis, neutropenia, anemia, and elevated AST, ALP, BUN, SC. Conclusively, given the sustained prevalence of bovine prioplasmosis and anaplasmosis in Chennai, control should integrate periodic PCR screening of high-risk crossbred cattle (targeted surveillance) with pre-monsoon/monsoon acaricide and anti-piroplasmal/anti-rickettsial intensification (region-specific prophylaxis). This integrated approach detects LM-missed positives, distinguishes similar-looking hemopathogens, and facilitates pathogen-specific therapy (anti-babesial, anti-theilerial, anti-anaplasmal), with targeted clearance of carriers (notably A. marginale), reducing treatment failure, drug misuse and transmission surges. However, these hospital data may be biased by repeat cases, warranting field validation.
Indexed as
Identifiers
42446826What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.