Evidence map›Paper›PMID 42446810›Full record

ArticleFish physiology and biochemistry2026

Transcriptomic analysis suggests FoxO and steroid hormone biosynthesis pathways are associated with 17α-methyltestosterone-induced partial masculinization in Trachinotus blochii.

Da Zheng, Huapeng Chen, Huan Liang, Jian Luo, Feibiao Song

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Article in Fish physiology and biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Da ZhengSchool of Marine Biology and Fisheries, Hainan Aquaculture Breeding Engineering Research Center, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou, 570228, China.
Huapeng ChenSchool of Marine Biology and Fisheries, Hainan Aquaculture Breeding Engineering Research Center, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou, 570228, China.
Huan LiangSchool of Marine Biology and Fisheries, Hainan Aquaculture Breeding Engineering Research Center, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou, 570228, China.
Jian LuoSchool of Marine Biology and Fisheries, Hainan Aquaculture Breeding Engineering Research Center, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou, 570228, China.
Feibiao SongSchool of Marine Biology and Fisheries, Hainan Aquaculture Breeding Engineering Research Center, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou, 570228, China. songfb@hainanu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated the effects of dietary 17α-methyltestosterone (MT) on gonadal differentiation and transcriptomic changes associated with partial masculinization in Trachinotus blochii. Larvae (3 days post-hatching) were fed a diet containing 100 mg/kg MT for 60 days. The results showed that MT treatment induced intersex gonadal development in 73% of genetic females, with gonads characterized by the presence of spermatogonia. Hormone assays showed increased MT levels and reduced 17β-estradiol (E2) levels in MT-treated intersex individuals compared with control females. Compared with control females, MT-treated intersex individuals showed increased expression of male-associated genes, including cyp11b1, sf1, and dmrt1, whereas cyp19a1a was reduced. However, other female-associated genes, such as hsd17β1 and foxl2, were not completely suppressed. Transcriptome profiling identified significant enrichment of FoxO-related and steroid hormone biosynthesis pathways. Trend analysis suggested that changes in oocyte meiosis-related and steroid hormone biosynthesis-related genes may be associated with MT-induced gonadal remodeling. These findings provide transcriptomic evidence for pathways potentially associated with MT-induced intersex gonadal development and may inform future studies on sex-control strategies in T. blochii, although fertility testing and reproductive validation of these intersex individuals are still required.

Indexed as

FishesForkhead Transcription FactorsMethyltestosteroneAnimalsDisorders of Sex DevelopmentFemaleGene Expression ProfilingMaleSex DifferentiationTranscriptomeForkhead Transcription FactorsMethyltestosteroneGonadal differentiationIntersex gonadMonosex breedingSteroidogenesis

Identifiers

PMID42446810

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